中国临床药理学杂志Issue(5)：438-441,4.
同分异构体补骨脂素和异补骨脂素对HepG2细胞及细胞色素 P450酶的影响
Impacts of isomers-psoralen and isopsoralen on HepG2 cells and cytochrome P450 enzymes
摘要
Abstract
Objective To observe the effects of psoralen and isopsoralen on cell proliferation , CYP gene expression , protein localization and pro-tein expression of HepG2 cells.Methods The effects on CYP1A1 and CYP3 A4 of psoralen and isopsoralen were tested by MTT colorimetry , flow cytometry determination , real time quantitative PCR technology , im-mune -fluorescence and protein immune -blot method.Results HepG2 cells were inhibited at gradient concentration by psoralen and isopsoralen.What′s more , there was a clear dose -response relation-ship .The two drugs ( 50 μg · mL-1 ) blocked HepG2 cells cycle at G2-M phase .The two drugs were CYP1 A1 and CYP3 A4 mRNA inhibi-tors but with different effects .Protein expression was significantly lower than the control group.The proliferation of HepG2 cells are inhibited by the two drugs , because the cell cycle was arrested at G 2 -M phase.Conclusion Compared with psoralen , isopsoralen respectively inhibited more on mRNA and protein expression of CYP3A4, but the intensity of inhibition of CYP1 A1 mRNA was slightly lower.关键词
补骨脂素/异补骨脂素/HepG2细胞/细胞色素P450Key words
psoralen/isopsoralen/HepG2 cell/cytochrome P450分类
医药卫生引用本文复制引用
..同分异构体补骨脂素和异补骨脂素对HepG2细胞及细胞色素 P450酶的影响[J].中国临床药理学杂志,2014,(5):438-441,4.基金项目
甘肃省技术研究与开发专项基金资助项目（1105TCYA024）；兰州市科技计划基金资助项目 （）
