中国临床药理学杂志Issue(6)：540-542,3.DOI:10.13699/j.cnki.1001-6821.2014.06.022
骨化三醇联合顺铂对人子宫内膜癌细胞株 HEC-1-A裸鼠移植瘤的作用
Effect of calcitriol combinating cisplatin on the HEC-1-A transplantation tumor of athymic mouse
摘要
Abstract
Objective To investigate the effect calcitriol combinating cisplatin on the human endometrial cancer ( HEC -1-A) transplanta-tion tumor of athymic mouse.Methods The HEC-1-A cell line was cultured in vitro.The thymic mouse with successfully tumor burden were divide into four groups:the control group (0.9%NaCl 0.25 mL), cal-citriol group (0.05 μg) , cisplatin group (50 μg) and combinating ( cal-citriol plus cisplatin ) therapy group.All of the four groups were treated once a week until 5 weeks.Results The difference of transplantation tumor volume between three therapy groups and control group is signifi-cant ( P<0.05 ).The apoptosis rate of the control , cisplati , calcitriol and combinating groups were ( 1.45 ±0.23 )%, ( 25.62 ±1.16 )%, (22.52 ±1.25 )%and (31.59 ±1.93 )%, respectively.There was sig-nificant statistical difference between combination group with the other three groups ( P<0.05 ).The mRNA expression level of Bcl -2 and Bax among the three therapy groups compared with control group were signifi-cant ( P <0.05 ).Conclusion Calcitriol had a strong anti -tumor effect in HEC-1-A transplantation tumor , the anti-tumor mechanism may associate with the Bax/Bcl-2 pathway induced HEC -1-A tumor cell apoptosis.关键词
子宫内膜腺癌/骨化三醇/顺铂/移植瘤/凋亡率Key words
adenocarcinoma endometrium/calcitriol/cisplatin/trans-plantation tumor/apoptosis rate分类
医药卫生引用本文复制引用
吴建波,郑秀,胡继芬,陈丽红,韦娟冰,陈一红..骨化三醇联合顺铂对人子宫内膜癌细胞株 HEC-1-A裸鼠移植瘤的作用[J].中国临床药理学杂志,2014,(6):540-542,3.基金项目
福建医科大学教授基金资助项目 （）
