中国临床药理学杂志Issue(8)：721-723,3.
高效液相色谱质谱联用法测定人血浆中万古霉素和去甲万古霉素浓度
Simultaneous determination of vancomycin and norvancomycin in human plasma by LC-MS/MS
摘要
Abstract
Objective To develop an LC -MS/MS method for simulta-neous determination of vancomycin and norvancomycin concentrations in human plasma.Methods The plasma samples were precipitated by 25%trichloroacetic acid -acetonitrile ( 2∶1 ).The separation was a-chieved on ZORBAX Extend C 18 column with gradient elution using water and acetonitrile ( 0.1% formic acid in both ).Detection of the analytes were made using positive ion electrospray ionization ( ESI) in the multi-ple reaction monitoring ( MRM) mode.Results The linear calibration curves of vancomycin and norvancomycin were both in the range of 0.2-100.0 μg· mL-1 ( r=0.997 8 ) , with the lower limit of quantitation at 0.2 μg· mL-1.The average recovery was 94.4%-107.2%, Intra-day and inter-day relative standard deviations were both below 15%.Conclusion This method is rapid , sensitive , accurate , specific , and reliable, suitable for simultaneous determination of vancomycin and nor-vancomycin concentration in human plasma.关键词
万古霉素/去甲万古霉素/液质联用法/血药浓度Key words
vancomycin/norvancomycin/LC -MS/MS/plasma con-centration分类
医药卫生引用本文复制引用
李贺,廉洪,肖昌钱,李力..高效液相色谱质谱联用法测定人血浆中万古霉素和去甲万古霉素浓度[J].中国临床药理学杂志,2014,(8):721-723,3.基金项目
国家科技重大专项基金资助项目（2013ZX09303005），国家自然科学基金资助项目 （）
