中国临床药理学杂志Issue(11)：1002-1005,4.DOI:10.13699/j.cnki.1001-6821.2014.11.008
高效液相色谱－四级杆飞行时间串联质谱的儿童性早熟代谢组学研究
Metabolomics study on central precocious puberty using HPLC -QTOF -MS analysis
王玲娜 1吴海瑛 2陈临琪 3汪维鹏3
作者信息
- 1. 苏州大学 药学院 药物代谢动力学研究中心，江苏 苏州 215123
- 2. 常州市药品检验所，江苏 常州 213002
- 3. 苏州大学 附属儿童医院 内分泌科，江苏 苏州 215003
- 折叠
摘要
Abstract
Objective To apply metabolomics studies to find out meta-bolic markers for further understanding the pathogenesis of children cen-tral precocious puberty ( CPP ).Methods A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrom-etry method ( HPLC-QTOF-MS) was used to figure out the metabolic characteristic of the CPP children and healthy children.Combined with the principal component analysis ( PCA) and partial least squares-diac-riminant analysis ( PLS-DA) , the major metabolic changes in peripher-al blood between the normal and onset group were statistically analyzed.According to the loading plot and variable importance plot ( VIP ) of PLS, twenty-four metabolic markers were identified.Furthermore, the pathways mediated by these metabolic markers were analyzed.Results CPP is closely related to the abnormalities of multiple metabolic path-ways, including amino acid, catecholamine, serotonin, and lipid metab-olism.Additionally, environmental endocrine disruptors play an impor-tant role in the occurrence of CPP.These findings provide a novel insight to the pathogenesis of CPP.Conclusion The metabolic characteristic of the CPP children was obviously different from that of the normal children.关键词
代谢组学/液相色谱-四级杆飞行时间质谱/性早熟Key words
metabonomics/high performance liquid chromatographycoupled with quadrupole time-of-flight mass spectrometry method/central precocious puberty分类
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王玲娜,吴海瑛,陈临琪,汪维鹏..高效液相色谱－四级杆飞行时间串联质谱的儿童性早熟代谢组学研究[J].中国临床药理学杂志,2014,(11):1002-1005,4.
