中国临床药理学杂志Issue(11)：1020-1022,3.DOI:10.13699/j.cnki.1001-6821.2014.11.013
霞草苷对人结肠癌HT－29细胞生长和凋亡的影响
Effects of Gypsophila oldhamiana gypsogenin on growth and apoptosis of human colon cancer HT-29 cells
摘要
Abstract
Objective To investigate the effects of Gypsophila oldhami-ana gypsogenin( GOG) on induction of apoptosis in human colon cancer HT-29 cells.Methods The growth and apoptosis of HT -29 cells treated with 5,10,20,40 μmol· L-1 GOG are investigated by MTT assay and flow cytometry.The mRNA expression of Caspase-3 and Bax was measured by real-time fluorescence quantitative RT-PCR.Results GOG inhibited the proliferation of HT -29 cell line in a dose -and time-dependent manner. Cell apoptosis rate increase obviously.Caspase-3 and Bax mRNA expression quantity increase gradually, assumes the obvious density dependence.Conclusion GOG could inhibit HT-29 cell proliferation through inducing cell apoptosis in vitro.The mechanism might be that GOG could enhance the level of Bax and activate the pathway of Caspase-3.关键词
结肠癌/霞草苷/细胞凋亡/半胱氨酸蛋白酶-3/Bcl-2相关X蛋白Key words
colon cancer/Gypsophila oldhamiana gypsogenin/cell apoptosis/caspase-3/Bcl-2 associated X protein分类
医药卫生引用本文复制引用
田桂红,秦迎,周玲,仲英,牟艳玲..霞草苷对人结肠癌HT－29细胞生长和凋亡的影响[J].中国临床药理学杂志,2014,(11):1020-1022,3.基金项目
国家自然科学基金资助项目 （）
