中国临床药理学杂志Issue(12)：1101-1104,4.DOI:10.13699/j.cnki.1001-6821.2014.12.008
康脑液对局灶性脑缺血再灌注大鼠的脑保护作用机制研究
Study mechanism of protective effects of Kangnao liquid on brain of rats with focal cerebral ischemic-reperfusion injury
摘要
Abstract
Objective To observe the effect of Kangnao liquid ( KNY) on the expression of basic fibroblast growth factor ( bFGF ) , phosphatidyli-nositol 3-kinase/protein kinase B ( Pi3k/akt) and microvascular densi-ty ( MVD) in rats with focal cerebral ischemia -reperfusion.Methods The male SD rats were randomly divided into six groups:three dose KNY groups [ 24 , 12 , 6 g · ( kg · d ) -1 ] , nimodipine group [1 mg· (kg· d) -1], model group, sham group.The animal models of middle cerebral artery occlusion ( MCAO) were established by a monofi-lament method from right internal carotid artery.Immunohistochemical method was used to investigate the expression of bFGF and Pi 3 k/akt in rats on 12,24,48,72,168 h of reperfution injury after ischemia for 2 h and to mark neovascular vWF after 72 h of reperfution and to calculate MVD.Results Compared with model group , the expression of bFGF , Pi3k/akt at different time points and MVD of brain tissue were signifi-cantly increased of KNY groups ( P<0.05 or P<0.01 ).Conclusion KNY can increase the expression of bFGF and Pi 3 k/akt , promote tissue angiogenesis , then protect nerve cells of cerebral infarction areas.关键词
康脑液/缺血再灌注/微血管密度/碱性成纤维细胞生长因子/磷脂酰肌醇-3-羟激酶/蛋白激酶B通路Key words
Kangnao liquid/cerebral ischemia reperfusion injury/micro-vascular density/basic fibroblast growth factor/phosphatidylinositol 3-kinase/protein kinase B pathway分类
医药卫生引用本文复制引用
肖志娟,赵志敏,邹玉安,薛茜,王静..康脑液对局灶性脑缺血再灌注大鼠的脑保护作用机制研究[J].中国临床药理学杂志,2014,(12):1101-1104,4.基金项目
河北省卫生厅课题基金资助项目 （）
