中国临床药理学杂志Issue(12)：1105-1107,3.DOI:10.13699/j.cnki.1001-6821.2014.12.009
解整合素－金属蛋白酶8在二乙基亚硝胺诱导的动物肝细胞癌过程中的表达分析
Expression analysis of a disintegrin and metalloprotease 8 during hepatocellular carcinoma induced by diethylnitrosamine in mice
摘要
Abstract
Objective To study the expressed dynamic change of a dis-integrin and metalloprotease 8 ( ADAM8 ) during hepatocellular carci-noma ( HCC ) induced by diethylnitrosamine ( DEN ) in mice.Methods Seventy mice were randomly divided into two groups:nor-mal group ( n=10 ) and experimental group ( n=60 ).The mice in experimental group were fed daily with 30 μg · mL -1 DEN in drin-king water for 24 weeks.Ten mice were respectively sacrificed at 4 , 8 , 12 , 16 , 20 and 24 weeks, then the livers were taken from the mice and divided into two sections.One was used to detect the occurrence of liver cancer by HE staining, the other was used to detect the ADAM8 expression by Western blot during HCC induced by DEN in mice.The normal control mice were also detected as experimental mice.Results HCC model was successfully induced at 24 weeks after DEN induction.The expression of ADAM8 was gradually increased and reached the peak level at 24 weeks after DEN induc-tion.Conclusion ADAM8 were remarkably differently expressed during HCC induced by DEN in mice.ADAM8 could play important roles in promoting the occurrence and development of HCC.关键词
二乙基亚硝胺/解整合素-金属蛋白酶8/肝细胞癌/表达分析Key words
diethylnitrosamine/a disintegrin and metalloprotease 8/hepa-tocellular carcinoma/expression analysis分类
医药卫生引用本文复制引用
李三强,崔旭红,蒙宏叶,马曌,韩红梅..解整合素－金属蛋白酶8在二乙基亚硝胺诱导的动物肝细胞癌过程中的表达分析[J].中国临床药理学杂志,2014,(12):1105-1107,3.基金项目
国家自然科学基金资助项目（81201558）；河南省高校科技创新人才支持计划基金资助项目（13HASTIT025）；河南省重点科技攻关基金资助项目（122102310030）；河南省教育厅科学技术研究基金重点资助项目 （）
