中国临床药理学杂志Issue(2)：110-112,3.DOI:10.13699/j.cnki.1001-6821.2015.02.009
质谱法对鲍曼不动杆菌的快速鉴定研究
Research of rapid mass spectrum identification of Acinetobacter baumannii
摘要
Abstract
Objective To establish the rapid identification of Acineto-bacter baumannii by detecting specific volatile metabolites of Acinetobacter baumannii through surface desorption atmospheric pressure chemical ioni-zation mass spectrometry ( SDAPCI-MS).Methods Ten clinical isola-ted Acinetobacter baumannii were resuscitated and vaccinated to blood agar plates at 37 ℃incubation and culturing.SDAPCI-MS was adopted to detect volatile metabolites of Acinetobacter baumannii by different cul-turing time.Results Compared with blank culture medium , the pecu-liar volatile metabolites were detected ,the characteristic peaks are mass -to-charge ratio 60 and 118.Basically the same volatile metabolites of Acinetobacter baumannii were generated by different culturing time.Con-clusion The rapid identification of Acinetobacter baumannii through peculiar volatile metabolites is hopefully applied by SDAPCI -MS.关键词
鲍曼不动杆菌/挥发性代谢产物/表面解吸常压化学电离质谱Key words
Acinetobacter baumannii/volatile metabolite/surface desorp-tion atmospheric pressure chemical ionization mass spectrometry分类
医药卫生引用本文复制引用
杭亚平,胡龙华,汪红,宁长秀,钟桥石,胡晓彦,章白苓,张楠,王小中..质谱法对鲍曼不动杆菌的快速鉴定研究[J].中国临床药理学杂志,2015,(2):110-112,3.基金项目
国家自然科学基金资助项目 （）
