中国临床药理学杂志Issue(2)：119-121,3.DOI:10.13699/j.cnki.1001-6821.2015.02.012
超高效液相色谱法测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度
Determination of phenytoin sodium, phenobarbital and carbamazepine in human serum by ultra performance liquid chromatography
摘要
Abstract
Objective To establish an ultra performance liquid chroma-tography ( UPLC) method for the determination of phenytoin sodium , phe-nobarbital and carbamazepine in human serum.Methods Serum sam-ples were injected and analyzed after protein precipitation with acetoni-trile.The analysis was performed on an ACQUITY UPLC BEH C 18 column, and the mobile phase consisted of acetonitrile -water (28∶72), the flow rate was 0.3 mL · min-1 , the detection wavelength was set at 220 nm and the temperature was maintained at 20 ℃.Results The peak area and content of phenytoin sodium , phenobarbital and carbama-zepine showed good linearity in the range of 2.5-80.0, 5.0-160.0 and 1.0-32.0 μg · mL-1 , respectively.Intra-and inter -day RSDs were all lower than 7.28%. The recoveries of phenytoin sodium were 96.18%-103.89%.Conclusion The established method is simple , accurate and rapid , and can be applied to determination of phenytoin sodium , phenobarbital and carbamazepine in human serum.关键词
苯妥英钠/苯巴比妥/卡马西平/超高效液相色谱法/血清Key words
phenytoin sodium/phenobarbital/carbamazepine/ultra performance liquid chromatography/serum分类
医药卫生引用本文复制引用
鲁静,陈天朝..超高效液相色谱法测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度[J].中国临床药理学杂志,2015,(2):119-121,3.基金项目
常州四药临床药学科研基金资助项目 （）
