中国临床药理学杂志Issue(3)：212-215,4.DOI:10.13699/j.cnki.1001-6821.2014.03.016
超高效液相串联质谱法测定大鼠西酞普兰及其代谢物的血药浓度
Determination the concentration of citalopram and its metabolites in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
Objective To develop an ultra performance liquid chroma-tography -tandem mass spectrometry method for the determination the concentration of citalopram and its metabolites ( citalopram N-oxide and N -desmethylcitalopram ) in rat plasma.Methods Plasmas were extracted with ethyl acetate.An ACQUITY UPLC BEH C18 ( 50 mm × 2.1 mm, 1.7 μm) column was used as the stationary phase.The mobile phase was consisted of acetonitrile and 0.1% formic acid water with gradient elution pumped at a flow rate of 0.4 mL· min-1.The analytes were detected with positive electrospray ionization in multiple reaction monitoring ( MRM ) mode and tramadol was used as internal standard.Results Excellent linear calibration curves of citalopram, N-desmethyl-citalopram and citalopram N-oxide were obtained in the concentration range of 0.1-10.0, 0.1-10.0 and 0.01-2.00 ng· mL-1 ( r=0.999 6, 0.999, 0.999 6).The lower limit of quantification were 0.05, 0.05 and 0.01 ng · mL-1.The average recovery was 96.09%-105.33%.The intra and inter day relative standard deviations were all less than 8.69%. Conclusion The method is simple, rapid and sensitive, which is suit-able for pharmacokinetics study of citalopram and its metabolites in rats.关键词
西酞普兰/血药浓度/药代动力学/超高效液相串联质谱法Key words
citalopram/plasma concentration/pharmacokinetic/ultra performance liquid chromatography-tandem mass spectrometry分类
医药卫生引用本文复制引用
吴春美,王双虎,胡国新,周云芳..超高效液相串联质谱法测定大鼠西酞普兰及其代谢物的血药浓度[J].中国临床药理学杂志,2015,(3):212-215,4.基金项目
卫生部行业科研专项基金资助项目 （）
