中国临床药理学杂志Issue(4)：289-291,3.DOI:10.13699/j.cnki.1001-6821.2015.04.016
高效液相色谱法测定人血浆中牛磺酸的浓度
Determination of taurine in human plasma by high performance liquid chromatography
台红祥 1高卫真 1范晓静 1李芹 1焦建杰 1娄建石 1张才丽1
作者信息
- 1. 天津医科大学药理学教研室，天津 300070
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chroma-tography ( HPLC ) method to determine taurine in human plasma. Methods Former derivatization method by DNFB pillar, with gradient elution of each component was adopted.The compounds were separated on a Kromasil C18 column, and mobile phase A: 0.05 mol・ L-1 CH3COONa solution ( pH 6.5 ) , mobile phase B: acetonitrile -water (1∶1). The flow rate was 1 mL・ min-1 , column temperature was 30 ℃, and the detection wavelength was 360 nm.Results The assay had the calibration range from 3.12 to 400.00μg・ mL-1 ( r>0.999 9 ) , and the intra-day RSD and inter-day RSD were all lower than 5%. The lowest limit of quantification was 3.12 μg・ mL-1 for taurine. Conclusion The method is simple, accurate, sensitive and specific for the determination of taurine in human plasma.关键词
高效液相色谱法/牛磺酸/血药浓度Key words
high performance liquid chromatography/taurine/plasma concentration分类
医药卫生引用本文复制引用
台红祥,高卫真,范晓静,李芹,焦建杰,娄建石,张才丽..高效液相色谱法测定人血浆中牛磺酸的浓度[J].中国临床药理学杂志,2015,(4):289-291,3.
