中国临床药理学杂志Issue(5)：370-374,5.DOI:10.13699/j.cnki.1001-6821.2015.05.015
高效液相色谱法、荧光偏振免疫分析法和质谱法监测丙戊酸血药浓度的比较
Comparison of high performance liquid chromatography, fluorescence polarization immunoassay and mass spectrometer method in determination of valproic acid in serum
赵明明 1孙亚欣 1邱枫 1徐英宏 1肇丽梅1
作者信息
- 1. 中国医科大学 附属盛京医院 临床药理研究室，沈阳 110004
- 折叠
摘要
Abstract
Objective To compare high performance liquid chromatog-raphy ( HPLC ) , fluorescence polarization immunoassay ( FPIA ) and mass spectrometer ( HPLC-MS) method for the determination of valproic acid in serum and to evaluate the correlations among three methods.Methods Two hundred and seven steady -state serum samples were collected from epileptic patients who had taken valproate.Three methods were employed to determine the concentration of valproic acid ( VPA ) and the results were analyzed to evaluate the correlation and difference.Results The results determined by three methods had high correlation.However, there was significant difference between HPLC and FPIA method.The concentration determined by FPIA was 0.7 μg · mL-1 higher than that of HPLC (95%CI:-8.6~9.9 μg· mL-1 ).When the concentration of valproic acid was less than 90μg· mL-1 , the two meth-ods appeared no significant difference.While the concentration of valpro-ic acid was more than 90μg· mL-1 , there was significant difference be-tween the two methods.Conclusion It is necessary to pay attention to the difference among three methods when monitoring the concentration of valproic acid in clinical practice.When the concentration was more than 90 μg· mL-1 , the results determined by HPLC and FPIA method could not be compared directly.关键词
丙戊酸/高效液相色谱法/荧光偏振免疫分析法/质谱法/相关性Key words
valproic acid/high performance liquid chromatography/fluorescence polarization immunoassay/mass spec-trometer/correlation分类
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赵明明,孙亚欣,邱枫,徐英宏,肇丽梅..高效液相色谱法、荧光偏振免疫分析法和质谱法监测丙戊酸血药浓度的比较[J].中国临床药理学杂志,2015,(5):370-374,5.
