中国临床药理学杂志Issue(7)：536-539,4.DOI:10.13699/j.cnki.1001-6821.2015.07.015
延伸因子2在肺动脉高压大鼠发病中的变化及葛根素的干预作用研究
Change of elongation factor 2 in pulmonary hypertension rats and study of interfered with Puerarin
摘要
Abstract
Objective To analysis the pulmonary tissue proteomic a-mong the normal , pulmonary hypertensive induced by monocrotaline and Puerarin rats , How the Puerarin inhibits the form of pulmonary hyperten-tion and pneumoangiogram rebuilding cleared.Methods SD ras random-ly divided into 5 groups, the normal group, the solvent group, the model group in two times, monocrotaline and puerarin group ( experimental group ) , 10 in each group .The solvent group was intraperitoneal injected of ethanol mixed with 0.9% sodium chloride solution ( 50 mg · kg -1 weight ) every morning and bred three weeks .The model group was intra-peritoneal injected monocrotaline (50 mg· kg -1 weight)once and respec-tively bred 2 or 3 weeks.The experimental group, after the injection of monocrotaline solution once , was intraperitoneal injected puerarin liquid (40 mg· kg -1 weight ) every morning in three weeks .Set up the pulmo-nary hypertensive rat model sucessfully , two dimensional gel electropho-resis of the total proteins from these five group , gel enzymolysis.Matrix-assisted laser desorption ionization -time of flight mass spectrometry ( MALDI-TOF-MS) and retrieval databank in NCBInr and discuss the results.Results The pulmonary hypertention , pneumoangiogram rebuil-ding , the right ventricle hypertrophy was inhibited by puerarin.The MALDI-TOF-TOF-MS discovered that the elongation factor 2 express very little in normal group and express a great quantity in 3-week-monocrotaline group , but in puerarin group ex-press down quickly.Conclusion The puerarin could inhibit the form of pulmonary hypertention by interfering in elon-gation factor 2 , it worth to study more about it.关键词
葛根素/延伸因子2/野百合碱/肺动脉高压/双向凝胶电泳Key words
puerarin/elongation factor 2/monocrotaline/pulmonary hypertension/two-dimensional gel electrophoresis分类
医药卫生引用本文复制引用
吴军,王良兴,龚玉强,施孟如,王金丹..延伸因子2在肺动脉高压大鼠发病中的变化及葛根素的干预作用研究[J].中国临床药理学杂志,2015,(7):536-539,4.基金项目
温州市科技计划基金资助项目 （）
