中国临床药理学杂志Issue(7)：554-556,3.DOI:10.13699/j.cnki.1001-6821.2015.07.020
血糖波动对成骨细胞的损伤机制研究
Mechanism of blood glucose fluctuation for the damage of mouse osteoblast cells
摘要
Abstract
Objective To observe the effect of blood glucose fluctuation and sustained high blood glucoseon on expression of mouseosteoblast cells of β-catenin, CyclinD1 gene.Methods Mouseosteoblast cells were cultured in vitro and divided into 3 groups, normal glucose ( 5.5 mmol · L-1 ) , high glucose ( 25.0 mmol · L-1 ) group , glucose fluctua-tion(5.5~25.0 mmol· L-1 ) group.The mice was intervented after a certain period of time , then the transcription levels of β-catenin and CyclinD1 mRNAs were determined by RT-PCR.And the expressions of B-catenin and CyclinD 1 were determined by Western blot .Results High glucose and glucose fluctuation decreased the mRNA transcription and protein expression levels of β-catenin and CyclinD1 in dose-and time-dependent modes .Conclusion High glucose and glucose fluctua-tion inhibited the proliferation of mouseosteoblast cells by a possible mechanism of inhibiting the activity of Wnt/β-catenin signaling pathway .关键词
血糖波动/成骨细胞/细胞增殖/Wnt/β-catenin信号通路Key words
glucose fluctuation/osteoblast/cell proliferation/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
颜晓芳,袁欣,严孙杰,杨立勇..血糖波动对成骨细胞的损伤机制研究[J].中国临床药理学杂志,2015,(7):554-556,3.基金项目
福建省教育厅基金资助项目 （）
