中国临床药理学杂志Issue(8)：635-639,5.DOI:10.13699/j.cnki.1001-6821.2015.08.016
藏药独一味中的主要化学成分环烯醚萜苷化合物在动物的镇痛抗炎作用研究
Analgesic and anti -inflammatory activities of iridoid glycosides from Tibetan medicine Lamiophlomis rotata in Balb/C mice
摘要
Abstract
Objective To study the analgesic and anti -inflammatory ac-tivities of iridoid glycoside from Lamiophlomis rotata in mice . Methods After one dose of iridoid glycosides ( 200 mg・ kg -1 ) , 8 -O-acetylshanzhiside methylester , 8 -deoxyshanzhiside , sesamoside , 7 ,8-dehydropenstemoside , and shanzhiside methylester , were adminis-tered by tail vein injection .Morphine ( 5 mg・ kg -1 ) and aspirin ( 100 mg・ kg-1 ) were used as positive controls , and 0.9% NaCl as negative control.The hot plate test , acetic-acid induced writhing test , formalin test , carrageenan -induced paw edema test and xylene -induced ear swelling test , peritoneal capillary permeability test on mice were used to test the analgesic and anti-inflammatory activities.Results Compared with blank group , these five iridoid glycosides could remarkably induce the number of writhing reaction with the inhibition ratio were 43.96%, 47.81%, 25.96%, 37.70%, and 26.23% ( P <0.01 , P <0.01 , P<0.05 , P<0.01 , and P<0.05 );could inhibit the xylene -induced ear swelling were 0.79%( P<0.05 ) , 43.07% ( P<0.01 ) , 7.90%, 14.25%, and 5.59%; exhibited significant analgesic activities on the second period of formalin test by 8 -O -acetylshanzhiside methylester and 8-deoxyshanzhiside ( P<0.01 ) .All compounds exhibited effects on paw edema induced by carrageenan in 1 hour, but only 8-O-acetylshanzhiside methylester and 8-deoxy-shanzhiside were effective in 3 hours with inhibition ratio were 32.84%and 40.36%, respectively.They also reduced permeability of ab-dominal capillaries and inflammatory peritoneal exudation induced by acetic acid , especial for 8-O-acetylshanzhiside methylester, 8-deoxyshanzhiside and sesamoside.On the other hand, these iridoid glycosides couldn′t prolong the leg-licking time in hot plate test and the first period of formalin test .Conclusion The five iridoid glycosides , espicially 8-O-acetylshanzhiside methylester and 8-deoxyshanzhiside exhibited significant analgesic and anti -inflammatory activities.关键词
环烯醚萜苷/8-去羟基山栀子苷/镇痛/抗炎Key words
iri doid glycosides/8-deoxyshanzhiside/analgesic/anti-inflammatory分类
医药卫生引用本文复制引用
贾诩,李茂星,陶锐,傅仲学..藏药独一味中的主要化学成分环烯醚萜苷化合物在动物的镇痛抗炎作用研究[J].中国临床药理学杂志,2015,(8):635-639,5.基金项目
国家科技部重大新药创制基金资助项目（2008ZXJ09004-52）；甘肃省科技重大专项基金资助项目（1102FKDA012）；甘肃省中医药管理局科研课题基金资助项目 （）
