中国临床药理学杂志Issue(9)：718-721,4.DOI:10.13699/j.cnki.1001-6821.2015.09.012
多重耐药基因多态性对肾移植患者他克莫司血药浓度／校正剂量比值的影响
Effect of multidrug resistance gene polymorphism on tacrolimus plasma concentration in Han population receiving renal transplant
摘要
Abstract
Objective To investigate the gene frequency and allele fre-quency of multidrug resistance gene ( MDR1) C1236T, G2677T/A and C3435T of Chinese renal transplant recipients of ethnic Han in Sichuan, and explore the relevance between the gene polymorphisms on tacrolimus and the ratio of blood concentration /dosage in the patients, so as to pro-vide reliable information for personalized tacrolimus administration in kid-ney transplanted patients by pharmacogenomics.Methods Totally 101 healthy volunteers and 56 patients receiving parental kidney transplanta-tion with above 6 -month follow -up were recruited, and gene chips were used to detect the single nucleotide polymorphism ( SNP) of MDR1 C1236T, G2677T/A and C3435T, with randomly selected from 50 sites for DNA sequencing to verify the method of gene chip.To determine the empty stomach blood concentrations of tacrolimus for 56 patients on the 7th, 14th and 30th day, and in the 3rd and 6th month after operation, and then compare the differences of various genotypes in the period of tacroli-mus blood concentration/dosage ratio.Results In the healthy volun-teers, the mutation frequency of MDR1G2677T/A, G2677T/A, C3435T was 43.05%, 14.90%, 40.10% respectively.These alleles frequency mutation resembles 56 cases of renal transplantation patients, which conforms to the Hardy-Weinberg equilibrium by the chi-square analysis.MDR1C1236T, G2677T/A and C3435T polymorphism within 6 months after renal transplan-tation at different time points tacrolimus blood concentration /dose ratio correction difference was not statistically signi-ficant ( P >0.05 ).Conclusion MDR1C1236T, polymorphism G2677T /A and C3435T and kidney transplant patients taking tacrolimus C /D ratio was no significant correlation.关键词
MDR1/基因多态性/他克莫司/血药浓度Key words
multidrug resistance gene/genetic polymorphism/tacrolimus/blood concentration分类
医药卫生引用本文复制引用
何霞,李翔,杨勇,张远,喻冬柯,王轶,童荣生..多重耐药基因多态性对肾移植患者他克莫司血药浓度／校正剂量比值的影响[J].中国临床药理学杂志,2015,(9):718-721,4.基金项目
四川省科技基础条件平台基金资助项目（4010159，2010147）；四川省卫生厅基金资助项目 （）
