中国临床药理学杂志Issue(9)：738-740,3.DOI:10.13699/j.cnki.1001-6821.2015.09.018
增殖细胞核抗原在小鼠肝细胞癌发生中表达的变化及意义
Expression change and significance of proliferating cell nuclear antigen in mice hepatocellular carcinoma
摘要
Abstract
Objective To investigate the dynamic change and signifi-cance of proliferating cell nuclear antigen ( PCNA ) expression of mice which were induced into hepatocellular carcinoma ( HCC ) by diethylni-trosamine ( DEN ) .Methods Mice continuously induced cancer with DEN containing drinking water for 24 weeks .The mice were killed in the induction of cancer at different time points.The expression of PCNA protein and nucleic acid in mice liver with HCC were evaluated by Western blot and reverse transcription polymerase chain reaction,respec-tively.Results The expressions level of PCNA protein and nucleic acid in the liver tissue in mice after induced into HCC by DEN increased gra-dually.They reached a peaked at 24th week, and there was significant differences between them ( P<0.05 ).Conclusion There is a signifi-cant dynamic change of PCNA expression in the liver tissue in mice during the progression of HCC which were induced by DEN.It suggest that PCNA might play an important role in the incidence and development of HCC.关键词
肝细胞癌/增殖细胞核抗原/二乙基亚硝胺Key words
hepatocellular carcinoma/proliferating cell nuclear antigen/diethylnitrosamine分类
医药卫生引用本文复制引用
马曌,李三强,徐正顺,吴秦川,胡景峰,付指辉,韩红梅,卢华杰..增殖细胞核抗原在小鼠肝细胞癌发生中表达的变化及意义[J].中国临床药理学杂志,2015,(9):738-740,3.基金项目
国家自然科学基金资助项目（81201558）；河南省高校科技创新人才支持计划基金资助项目（13HASTIT025）；河南省重点科技攻关基金资助项目（122102310030）；河南省教育厅科学技术研究基金重点资助项目 （）
