中国临床药理学杂志Issue(10)：858-860,3.DOI:10.13699/j.cnki.1001-6821.2015.10.028
脱嘌呤／脱嘧啶核酸内切酶／氧化还原因子-1基因与乳腺癌细胞对吉西他滨耐药的相关性研究
Relationship on chemoresistance between the apurinic/apyrimidinic endonuclease/redox factor-1 in breast cancer with induction of gemcitabine
摘要
Abstract
Objective To investigate the changes of apurinic/apyrimidi-nic endonuclease/redox factor -1 ( APE/Ref -1 ) expression in human breast cancer cell line MCF -7 after induction of gemcitabine and to study the effects of gemcitabine on chemoresistance in breast cancer . Methods After human breast cancer cell line MCF -7 was incubated with 50 nmol・ L-1 concentration of gemcitabine for 0,3,7,10,13 and 16 days, the apoptosis ratio was determinated by flow cytometry .And the mRNA and protein levels of APE/Ref -1 of survival cells were deter-mined by reverse transcription -polymerase chain reaction ( RT-PCR) and Western blot , respectively.Results After human breast cancer cell line MCF-7 was incubated with 50 nmol・ L -1 gemcitabine for 0,3,7, 10,13 and 16 days,the expression of APE/Ref-1 in human breast can-cer cell line MCF-7 was elevated significantly both at mRNA and pro-tein levels ,and were positively correlated with incubation time , which is consistent with the proportion of apototic cells .And the expression of APE/Ref-1 was increasing significantly from tenthdays , reaching the highest in sixteenth days .And when the drug resistant cells was com-pared with the non -drug resistant cells , which were both incubated with 50 nmol・ L-1 gemcitabine for 10,13 and 16 days.The expression of mRNA and protein of APE/Ref-1 in drug resistant cells have statistical significance ( P<0.05 ).Conclusion The obvious up -regulated expression of APE/Ref-1 may be an adaptive re-sponse contributing to the overall chemoresistance in breast cancer .关键词
吉西他滨/脱嘌呤/脱嘧啶核酸内切酶/氧化还原因子-1/乳腺癌/化疗耐药性/耐药Key words
gemcitabine/apurinic/apyrimidinic endonuclease/redox factor-1/breast cancer/chemoresistance/drug resistance分类
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郑敏,曹永晋,蔡伟华..脱嘌呤／脱嘧啶核酸内切酶／氧化还原因子-1基因与乳腺癌细胞对吉西他滨耐药的相关性研究[J].中国临床药理学杂志,2015,(10):858-860,3.基金项目
福建省卫生厅青年科研基金资助项目 （）
