中国临床药理学杂志Issue(12)：1143-1145,3.DOI:10.13699/j.cnki.1001-6821.2015.12.020
解整合素－金属蛋白酶9在对乙酰氨基酚诱导的小鼠急性肝损伤中的表达分析
Expression analysis of a disintegrin and metalloprotease 9 during in the mice induced by acetaminophen
摘要
Abstract
Objective To study the expressed dynamic change and roles of a disintegrin and metalloprotease 9 ( ADAM9) during acute liver injury induced by acetaminophen.Methods Fifty mice were randomly divided into two groups:normal group (n=10) and experiment group (n=40). The mice in experiment group were respectively drawn blood by removing the eyeballs and serum was separated to detect the activity of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) at 6, 24, 42 and 72 h after intraperitoneal injection of acetaminophen 550 mg・ kg-1 .The activity of serum AST and ALT in the mice of normal group were also detected.The expression of hepatic ADAM9 at protein and mRNA levels were detected by western blot and RT -PCR method in the mice of normal group and experiment group at different time points after acetaminophen injection.Results The expression of ADAM9 protein and mRNA were significantly down -regulated at 6 h ( P<0.05) , and the expression of ADAM9 protein and mRNA reached the lowest level at 24 h ( P <0.05 ) . After that, the expression of ADAM9 protein and mRNA were gradually increased.The expression of ADAM9 was significantly increased at 42 h (P<0.05),and then recovered closed to the normal level at 72 h after acetaminophen injection.Conclusion ADAM9 is remarkably differently ex-pressed during acute liver injury induced by acetaminophen, which indicates that ADAM9 may play an important pro-tective role during acute liver injury induce by acetaminophen in mice.关键词
对乙酰氨基酚/解整合素-金属蛋白酶9/急性肝损伤/表达分析Key words
acetaminophen/a disintegrin and metalloproteases 9/acute liver injury/expression analysis分类
医药卫生引用本文复制引用
李三强,韩红梅,卢华杰,席守民..解整合素－金属蛋白酶9在对乙酰氨基酚诱导的小鼠急性肝损伤中的表达分析[J].中国临床药理学杂志,2015,(12):1143-1145,3.基金项目
国家自然基金资助项目（ U1204802），河南省高校科技创新人才支持计划基金资助项目（13 HASTIT025），河南省重点科技攻关基金资助项目（122102310030），河南省教育厅科学技术研究重点基金资助项目 （）
