中国临床药理学杂志Issue(12)：1146-1149,4.DOI:10.13699/j.cnki.1001-6821.2015.12.021
促红细胞生成素对缺氧缺血性脑损伤新生大鼠脑组织 Fas／FasL表达的影响
Effect of erythropoietin on Fas/FasL expression in brain tissues of neonatal rats with hypoxic ischemic brain damage
摘要
Abstract
Objective To investigate the effect of erythropoietin ( EPO) on factor -related apoptosis and its ligand ( Fas/FasL ) expression in brain tissues of neonatal rats with hypoxic ischemic brain damage ( HIBD ) .Methods One hundred and twenty newborn rats ( 7 days years old ) were selected and randomly divided into 3 groups: sham group, hypoxic ischemic brain damage group ( model group ) and EPO treatment group ( test group ) , each group was further divided into five time points:6, 12, 24, 48 and 72 h after HIBD model was established. Eight rats in each group were killed at different time points.After which samples of brain tissues were harvested.Change of brain cell morphology was observed by HE staining and expression of Fas/FasL was observed by immunohistochemical staining.Results In model group, expressions of Fas and FasL reached the peak at 48 h after HIBD model was established and decreased at 72 h.Compared with sham group, the expressions of Fas and FasL in model group at each time point were much higher, and the difference was statistically significant ( P <0.05 ) .In test group, expressions of Fas and FasL at each time point were significantly lower than in model group ( P <0.05 ) .Conclusion Cell apoptosis in the brain tissue after hypoxic -ischemic damage could be remarkably inhi-bited by EPO through reduction of Fas and FasL expression in brain tissue of neonatal rats with HIBD.关键词
促红细胞生成素/缺氧缺血性脑损伤/凋亡相关因子/凋亡相关因子配体Key words
erythropoietin/hypoxic ischemic brain damage/factor-related apoptosis/factor-related apoptosis ligand分类
医药卫生引用本文复制引用
张晶,黄蕊,郝玲,刘娜,代少娟..促红细胞生成素对缺氧缺血性脑损伤新生大鼠脑组织 Fas／FasL表达的影响[J].中国临床药理学杂志,2015,(12):1146-1149,4.基金项目
河北省医学科学研究重点课题计划基金资助项目 （）
