摘要
Abstract
Objective To investigate the influence on proliferation, apoptosis and cell cycle of gastric cancer cell lines SNU-1 induced by molecular targeted drug sorafenib and lapatinib combined with ammonium pyrrolidinedithiocarbamate ( PDTC).Methods Groups were divided as follows:blank group ( A group ) , fore test groups, include B group, sorafenib+lapatinib(3,3μmol・ L-1, respectively),C group, sorafenib+PDTC(3,50 μmol・ L-1,respectively),D group, lapatinib +PDTC(3, 50 μmol・ L-1 , respectively ) , and E group, sorafenib +lapatinib +PDTC(3,3,50 μmol・ L-1 ,respectively) and three different concentra-tions of sorafenib and lapatinib ( 1.5 , 6.0 , 12.0 μmol・ L-) , PDTC (25,100,200 μmol・ L-1 ) as control groups, respectively.The inhibi-tion of gastric cell line SNU-1 was detected by MTT.The cell cycle and apoptosis were disclosed by flow cytometry, respectively.Results There were some certain inhibition of gastric cancer cell line SNU-1 induced by different groups.The differences had significantly in three drugs combi-nation groups on the rate of cell inhibition, the S-phase cells decreased, and increased apoptosis ( all P <0.05 ) . Conclusion Combining with three drugs can effectively inhibit the proliferation and induce apoptosis of gastric cancer cell SNU-1.关键词
靶向药物/索拉菲尼/拉帕替尼/吡咯烷二硫代氨基甲酸盐Key words
molecular targeted drug/sorafenib/lapatinib/ammonium pyrrolidinedithiocarbamate分类
医药卫生
