中国临床药理学杂志Issue(12)：1176-1179,4.DOI:10.13699/j.cnki.1001-6821.2015.12.029
高效液相色谱法测定人血浆中拉莫三嗪、奥卡西平及其代谢物浓度
Determination of concentrations of lamotrigine, oxcarbazepine and its active metabolite in human serum by HPLC method
摘要
Abstract
Objective To establish an HPLC method to determinate the concentrations of lamotrigine , oxcarbazepine and its active metabolite simultaneously in human serum. Methods Phenacetin was used as internal standard, and the plasma was extracted by diethyl ether.The HPLC method was performed on the C18 column. The mobile phase consisted of acetonitrile -0.05 mol・ L-1 biphosphate sodium ( 25∶75, pH 4.5).The flow rate was 1.0 mL・ min -1 and the column temperature was kept at 25 ℃.The detecting wavelength was set at 220 nm.The method was inspected in the aspects of specificity, lower limit of quantifi-cation, precision, recovery rate and stability.Results The linear range of lamotrigine, monohydroxycarbazepin and oxcabazepine were from 0.1 to 20.0 μg・ mL-1 ( y=20.11 ×10 -2 c +1.31 ×10 -2 , r =0.999 0 ) , 0.5-50.0 μg・ mL-1 ( y=9.77 ×10 -2 c+0.35 ×10 -2 , r=0.999 8 ) , 0.25 -25.0 μg・ mL-1 ( y =131.69 ×10 -2 c -0.59 ×10 -2 , r=0.999 1) , respectively.The range of extraction recovery ratios were both between 88.65%and 100.38%.The intra and inter-day precision were both less than 15%.Conclusion The method is shown to be sim-ple, accurate and sensitive, which can be applied in the determination of concentration of lamotrigine, oxcarbazepine and its active metabolite in human plasma.关键词
拉莫三嗪/奥卡西平/10-羟基卡马西平/高效液相色谱法/血药浓度Key words
lamotrigine/oxcarbazepine/monohydroxycarbazepin/HPLC/plasma concentration of drug分类
医药卫生引用本文复制引用
徐善森,张媞,周敬凯,刘美,肇丽梅..高效液相色谱法测定人血浆中拉莫三嗪、奥卡西平及其代谢物浓度[J].中国临床药理学杂志,2015,(12):1176-1179,4.基金项目
国家自然基金资助项目（81302857）；辽宁省自然科学基金资助项目 （）
