中国临床药理学杂志Issue(12)：1180-1183,4.DOI:10.13699/j.cnki.1001-6821.2015.12.030
HPLC－MS／MS法同时测定肝微粒体孵育体系中地西泮及其代谢产物的浓度
Determination of diazepam and its metabolites in human liver microsomal incubation systems by HPLC-MS/MS method
徐亚飞 1董瑞华 2曲恒燕 1王静 1刘泽源 1马静洁1
作者信息
- 1. 军事医学科学院 附属医院 临床药理学研究室，北京 100071
- 2. 延边大学药学院，吉林延吉 133000
- 折叠
摘要
Abstract
Objective To establish a HPLC-MS/MS method for simul-taneous determination of diazapam and its three metabolites in human liv-er microsomal.Methods Kromasil C18 column was used at 40 ℃, and the mobile phase was a mixed system consisting of acetonitrile -2 mmol・ L-1 mammonium acetate ( 70 ∶30 ) and a flow rate of 0.3 mL・ min -1 .The detection of the samples was made using positive ion scan and multiple reaction monitoring mode.The specificity, linearity and limit of quantification, precision and recovery, matrix effect and sta-bility were assessed.Results The sample showed good linearity from 20-1000 ng・ mL-1 ( r>0.99 ) and the lower limit of quantitation was 20 ng・ mL-1 .The liner calibration curve of nordiazepam, temazepam and oxazepam obtained concentration range of 2 -100 ng・ mL-1 (r>0.99) and the low limit of quantitation was 2 ng・ mL-1 .The average recovery rates of diazepam, nordiazepam, temazepam and oxaze-pam were 55.47% to 68.76%, 64.84% to 79.11%, 68.32% to 78.27%and 72.66%to 83.82%.The intra-day and inter-day relative standard deviations were both less than 15%.Conclusion The method is simple, rapid and accurate, suitable for measuring the concentration of diazapam and its three metabolites in human liver microsomal.关键词
高效液相色谱-质谱联用法/地西泮/去甲地西泮/替马西泮/奥沙西泮Key words
HPLC-MS/MS/diazapam/nordiazepam/temazepam/oxazepam分类
医药卫生引用本文复制引用
徐亚飞,董瑞华,曲恒燕,王静,刘泽源,马静洁..HPLC－MS／MS法同时测定肝微粒体孵育体系中地西泮及其代谢产物的浓度[J].中国临床药理学杂志,2015,(12):1180-1183,4.
