中国临床药理学杂志Issue(14)：1452-1454,3.DOI:10.13699/j.cnki.1001-6821.2015.14.029
高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度
Determination of cefazolin and ceftriaxone in Beagle dog plasma by high performance liquid chromatography
李天云1
作者信息
- 1. 北京大学 第一医院 临床药理研究所，北京100034
- 折叠
摘要
Abstract
Objective To develop a HPLC method for the determination of cefazolin and ceftriaxone in vivo.Methods Ceftriaxone use Phenome-nex Gemini C18 (4.6 mm ×150 mm, 5 μm) and cefazolin use ES indus-tries EpicC18 (4.6 mm ×150 mm, 5 μm) columns,all were used at 30℃.Cefazolin with a mobile phase of( pH=4.3 ) 0.05 mol・ L-1 ammo-nium acetate -methanol solution ( 70∶30 ) , ceftriaxone with a mobile phase of ( pH =6.0 ) 0.05 mol・ L-1 phosphater buffer -acetonitrile (94∶6).All at a flow-rate of 1.0 mL・ min -1 .Injection volume of 40μL all were used .Quantitaion was performed using HPLC.Results The straight line corresponding to equation were obtained:y=-1.25 ×104 x+8479.37( r=0.997 4 ) in the range of 2 -300 μg・ mL-1 for cefazolin;and y=1.25 ×104 x+2.72 ×104 ( r=0.997 6) in the range of 2-200μg・ mL-1 for ceftriaxone. The extraction recovery for cefazolin and ceftriaxone were ≥ 85.78% .The intra and inter day precision were ≤10%.Conclusion The analytical method appeared to be accu-rate, sensitive and convenient for the determineation of cefazolin and ceftriaxone in Beagle dog.It is suitable for animal pharmacokinetic study of cefazolin and ceftriaxone or in other fields.关键词
高效液相色谱法/头孢唑啉/头孢曲松/比格犬/血浆Key words
HPLC/cefazolin/ceftriaxone/Beagle dog/plasma分类
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李天云..高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度[J].中国临床药理学杂志,2015,(14):1452-1454,3.
