中国临床医学Issue(3)：239-241,3.
干扰素α对肝癌细胞中转录因子Sp1的表达及活性的影响
Effect of Interferon αon the Expression and Activity of Transcription Factor Sp 1 in Hepatocarcinoma Cells
摘要
Abstract
Objective:To evaluate the effect of interferon-αon the expression and activity of transcription factor specificity pro-tein 1(Sp1) in hepatocarcinoma cells .Methods :The effect of interferon-αon the expression and phosphorylation level of Sp1 in hepatocarcinoma cell line M HCC97H were detected by Western blotting .The effect of interferon-α on the activity of Sp1 was analyzed by electrophoretic mobility shift assay (EMSA) .Results :Interferon-α significantly reduced the expression and phos-phorylation level of Sp1 ,and the reducement on phosphorylation was more distinct .Interferon-α also inhibited the activity of Sp1 significantly .Conclusions :Interferon-αinhibits the activity of Sp1 by down regulating the expression and phosphorylation level of Sp1 .Sp1 may be an optional predictor of clinical efficacy of interferon-αon hepatocarcinoma .关键词
肝癌/转录因子/S p1/干扰素αKey words
Hepatocarcinoma/T ranscription factor/Sp1/Interferon-α分类
医药卫生引用本文复制引用
潘奇,陈万勇,朱凯,张巨波,赵一鸣,朱小东,孙惠川,王鲁,任宁..干扰素α对肝癌细胞中转录因子Sp1的表达及活性的影响[J].中国临床医学,2014,(3):239-241,3.基金项目
国家自然科学基金项目（编号30600589、30801102）；上海市科技启明星跟踪计划项目 （）
