中国临床医学Issue(3)：259-261,3.
microRNAs 在脂多糖诱导的结肠癌细胞炎性反应模型中的表达
The Expression of MicroRNAs in HT-29 Cells with Lipopolysaccharide-Induced Inflammation
吴海波 1才志刚 1张绍明 1张珩 1汪雷 1徐小平1
作者信息
- 1. 中国人民解放军第四五五医院心胸外科，上海 200052
- 折叠
摘要
Abstract
Objective:To study the expression of microRNAs (miRNAs) in colon adenocarcinoma cell line HT-29 with lipopo-lysaccharide-induced(LPS-induced) inflammation .Methods :HT-29 cells were treated with 400 ng/mL LPS for 48 h to establish inflammatory HT-29 cell model (LPS group) ,and HT-29 cells without LPS treatment were viewed as the control group .Total RNA from the two groups was isolated with Trizol reagent ,and IL-6 and CRP were examined .The expression of miRNAs was determined by Real-time PCR .Results:Compared with the control group ,the mRNA levels of IL-6 and CRP were up-regulated in LPS group .The expression levels of miR-22 ,miR-26 ,miR-214 in LPS group were lower than those in the control group , while miR-23 level was higher than that in the control group .The levels of miR-24 ,miR-30 and miR-181 were not statistically different between the two groups .Conclusions :MiRNAs may play important roles in LPS-induced inflammation of adenocarci-noma cells .关键词
microRNA/HT-29/炎性反应/脂多糖Key words
MicroRNA/HT-29/Inflammation/Lipopolysaccharide分类
医药卫生引用本文复制引用
吴海波,才志刚,张绍明,张珩,汪雷,徐小平..microRNAs 在脂多糖诱导的结肠癌细胞炎性反应模型中的表达[J].中国临床医学,2014,(3):259-261,3.
