中国临床医学Issue(6)：643-646,4.
小片段干扰 RNA 抑制人肝癌细胞株中YAP 基因表达的研究
Study on the Inhibitory Effect of Small Interfering RNA on Yes-Associated Protein Expression in Human Hepatoma Cell Lines
摘要
Abstract
Objective:To investigate the inhibitory effect of small interfering RNA(siRNA)on yes-associated protein(YAP) expression and adriamycin(ADM)chemosensitivity of hepatoma cell lines.Methods:The siRNA sequences were designed based on YAP gene and were transfected into four different kinds of hepatoma cells (PLC/PRF/5,Huh-7,MHCC 97H,MHCC 97L) through lipofectamineTM 2000.The inhibition result of YAP expression in hepatoma cells was measured by Western blotting and fluorescence microscope.The viability rate of hepatoma cells was measured by methyl thiazolyl tetrazolium(MTT)colori-metry after treating with different concentrations of ADM.Results:The siRNA expression vector to aim directly at YAP gene was constructed successfully.Western blotting revealed that transfection with siRNA could inhibit the expression of YAP pro-tein in hepatoma cell lines.The expression of YAP protein in hepatoma cell lines was specially and effectually inhibited by RNA interference(RNAi).MTT colorimetry detection result revealed that the viability rate of hepatoma cell lines significantly de-creased after transfection with YAP siRNA.Conclusions:Inhibiting the expression of YAP by siRNA can enhance the sensitivi-ty of hepatoma cell lines to ADM.关键词
小干扰 RNA/YAP/肝癌细胞/阿霉素Key words
Small interfering RNA/Yes-associated protein/Hepatoma cells/Adriamycin分类
医药卫生引用本文复制引用
张素青,陈橼,张一心,刘继斌,朱烨菁,钱俊,李德春..小片段干扰 RNA 抑制人肝癌细胞株中YAP 基因表达的研究[J].中国临床医学,2014,(6):643-646,4.基金项目
江苏省南通市社会发展科技计划项目 （）
