摘要
Abstract
Objective:To investigate the inhibitory effect of novel mTORC1/mTORC2 dual inhibitor OSI‐027 on human colon cancer cell line HT‐29 in vitro .Methods:HT‐29 cells ,cultured in vitro ,were either treated with different concentrations of OSI‐027(0 .1 ,1 ,10 ,25 and 50 nmol/L) ,or treated with 25 nmol/L OSI‐027 for 0 ,24 ,48 ,72 ,96 h ,respectively .Methyl thiazolyl tetrazolium assay(MTT) and cell colony forming assay were used to detect the growth and proliferation of HT‐29 cells after treatment with OSI‐027 .Flow cytometry (FACS) and trypan blue stalning were used to detect the influence of OSI‐027 on apoptosis and death of HT‐29 cells .Western blotting was used to detect the expression of apoptosis‐related proteins cleaved‐caspase‐3 and cytochrome C .Results:OSI‐027 inhibited the survival of HT‐29 cells ,and the inhibition was concentration and time dependent .It also inhibited the proliferation of HT‐29 cells ,and the inhibition was concentration dependent .It also induced apoptosis and death ,and the inducement was concentration dependent .Expression levels of apoptosis‐related proteins cleaved‐caspase‐3 and cytochrome C increased after OSI‐027 treatment .Conclusions:The novel mTORC1/mTORC2 dual inhibitor OSI‐027 can inhibit the proliferation of human colon cancer cell HT‐29 and induce cell apoptosis .关键词
结肠癌/OSI-027/哺乳动物雷帕霉素靶蛋白/凋亡/信号转导Key words
Colon cancer/OSI-027/Mammalian target of rapamycin/Apoptosis/Signal transduction分类
医药卫生
