中国临床医学Issue(2)：195-198,4.
丙酮酸乙酯对肝细胞氧化应激损伤的保护作用及其机制研究
Mechanism of Protection of Ethyl Pyruvate against Oxidative Stress Injury in Hepatocytes
王浩 1何义舟 1罗哲1
作者信息
- 1. 复旦大学附属中山医院麻醉科，上海 200032
- 折叠
摘要
Abstract
Objective:To investigate the protective effect of ethyl pyruvate on oxidative stress injury in hepatocytes and its underlying molecular mechanism .Methods:Hypoxia/reoxygenation‐induced oxidative stress injury model was performed with 1 h hypoxia followed by 1 h reoxygenation .According to the different interventions ,L‐02 hepatocytes were divided into control group(Con group) ,lactate Ringer’s group(LR group) and ethyl pyruvate group(EP group) .The concentrations of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in supernatants of cell culture medium were measured by Hitachi 7020 Automatic Analyzer (Hitachi ,Tokyo ,Japan) .The percentages of necrotic cells and apoptotic cells were examined by Hoechst 33342/propidium iodide (PI) and TdT‐mediated dUTP nick end labeling(TUNEL) stalning .The expression levels of caspase‐9 ,caspase‐3 and poly (ADP‐ribose) polymerase (PARP) were determined by Western blotting .The concentrations of ATP in hepatocytes were measured by using luciferase luminescence method .Results:The concentrations of AST and ALT in EP group were lower than that in Con group and LR group(P<0 .05) .The percentage of necrotic cells was lower and the percentage of apoptotic cells was higher in EP group than those in Con group and LR group(P<0 .05) .The expression levels of cleaved caspase‐9 ,caspase‐3 and PARP in EP group were higher than that in Con group and LR group(P< 0 .05) .The concentration of ATP in EP group was higher than that in Con group and LR group(P<0 .05) .Conclusions:Ethyl pyruvate can decrease the percentage of necrotic cells but increased the percentage of apoptotic cells in hepatocytes insulted by hypoxia/reoxygenation .Ethyl pyruvate may protect hepatocytes agalnst oxidative stress injury through up‐regulating the intracellular ATP levels and consequently skewing the necrosis‐apoptosis pathway .关键词
氧化应激损伤/坏死/凋亡/丙酮酸乙酯Key words
Oxidative stress injury/Necrosis/Apoptosis/Ethyl pyruvate分类
医药卫生引用本文复制引用
王浩,何义舟,罗哲..丙酮酸乙酯对肝细胞氧化应激损伤的保护作用及其机制研究[J].中国临床医学,2015,(2):195-198,4.
