中国临床医学Issue(3)：257-261,5.
膀胱感觉和运动神经支配的大鼠模型的建立及鉴定
Establishment and Identification of Rat Model with Reconstruction of Bladder Sensory and Motor Innervation
摘要
Abstract
Objective:To establish and identify a SD rat model with reconstruction of bladder sensory and motor innervation , so as to lay the foundation for further study of micturition center remodeling and its mechanisms .Methods:A total of 45 female SD rats were randomly divided into control group(n=10) ,rhizotomy group(n=15) and nerve root anastomosis group(n=20) . All the ventral and dorsal roots of spinal nerve below L4 level of rats in rhizotomy group were cut off .In nerve root anastomosis group ,the bilateral ventral and dorsal roots of L4 nerve ,were anastomosed with those of S1 nerve ,after the spinal nerve roots had been cut off .Rats in control group were not treated with surgery . At Six months after surgery ,rats in each group underwent urodynamic test ,nerve root stimulation ,toluidine blue staining at nerve anastomosis site ,pelvic ganglia fluorescence gold tracer staining and bladder wet weight measurements .Results:Bladder maximum capacity ,residual urine volume ,bladder compliance and bladder wet weight in nerve root anastomosis group was less than those in rhizotomy group ,however ,larger than those in control group(P<0 .05) .There was no statistically significant difference in maximum voiding pressure between nerve root anastomosis group and control group(P> 0 .05) ,however ,maximum voiding pressure in nerve root anastomosis group was larger than that in rhizotomy group(P<0 .05) .Intravesical pressure increased after nerve root stimulation in nerve root anastomosis group ,but it was still lower than that in control group(P<0 .05) .Nerve passing rate was (53 .4 ± 6 .7)% in nerve root anastomosis group ,under toluidine blue staining at nerve anastomosis site .After injection of fluorescent gold in pelvic ganglia ,fluorescence gold staining was visible in the L4 spinal cord gray matter in nerve root anastomosis group , however ,not visible in rhizotomy group and control group .Conclusions:SD rat model with reconstruction of bladder sensory and motor innervation is successfully established .It lays the foundation for further study of micturition center remodeling and its mechanism .关键词
尿流动力学/神经吻合/神经重塑/神经示踪/神经根电刺激Key words
Urodynamics/Nerve anastomosis/Neural remodeling/Nerve tracer/Nerve root stimulation分类
医药卫生引用本文复制引用
赵建国,雷德桥,蒙德鹏,侯春林,林浩东,宗海洋,陈寅生,蔡雨卫..膀胱感觉和运动神经支配的大鼠模型的建立及鉴定[J].中国临床医学,2015,(3):257-261,5.基金项目
国家自然科学基金资助项目 （）
