中国林副特产Issue(5)：8-11,12,5.
诱导食用真菌代谢生产木聚糖酶的研究
Screening of Xylanase-producing Edible Fungi by Xylan Induction
邢振楠 1臧海莲 2王丹丽 1范冬茹1
作者信息
- 1. 伊春林业科学院，黑龙江 伊春 153000
- 2. 东北农业大学
- 折叠
摘要
Abstract
Xylan as the inducing substrate ,using ultrathin-layer agar plate diffusion technique and DNS assay and rescreening ,eventually screened 3 xylanase producing high strains from 66 edible fungus in liq-uid fermentation induced culture .Black fungus LK8 ,Yutian mushroom ,M ushroom 236 have better en-zyme production capacity ,detection of single factor combined with orthogonal experiment finalized train-ing time ,pH and xylan concentration as the optimal condition for xylanase production were 20 d ,7 .5 and 1 .0 g/100mL ;25 d ,7 .0 and 1 .0 g/100mL ;25 d ,5 .0 and 0 .75 g/100mL ,respectively .Xylanase activi-ty under optimum conditions were :600 .6 IU/mL ,672 .5 IU/mL ,345 IU/mL .The culture solution pH value is the main factor affecting the induction effect .关键词
木聚糖酶/食用真菌/诱导代谢/液体发酵Key words
Xylanase/Edible fungi/Induction/Liquid fermentation引用本文复制引用
邢振楠,臧海莲,王丹丽,范冬茹..诱导食用真菌代谢生产木聚糖酶的研究[J].中国林副特产,2014,(5):8-11,12,5.
