中国免疫学杂志Issue(5)：587-590,4.DOI:10.3969/j.issn.1000-484X.2014.05.003
支原体脂肽经TLR2，6／c-Src／PI3 K通路诱导单核细胞表达血红素氧合酶-1
A mycoplasma lipopeptide induces hemeoxygenase-1 expression via TLR2, 6/c-Src/PI3 K pathway in monocytes
摘要
Abstract
Objective:To observe the molecular mechanism involved in expression of hemeoxygenase -1 (HO-1) induced by a macrophage-activating lipopeptide-2 (MALP-2).Methods:THP-1 cells were cultured in vitro and stimulated by MALP-2 for 12 h, expression of HO-1 was detected by Western blot .TLR2 and TLR6 neutralizing antibodies incubation , dominant negative plasmids transfection were used to assess the functional of TLR 2,6 in mediating HO-1 expression.Phosphorylation of c-Src and Akt were detec-ted by Western blot, and c-Src siRNA and PI3K inhibitor LY294002 were used to investigate the role of c-Src and PI3K in HO-1 ex-pression.Results:MALP-2 induced c-Src phosphorylation , and TLR2 and TLR6 neutralizing antibodies , or their dominant negatively plasmids could abrogate this effect .In addition, siRNA of c-Src could decrease the phosphorylation level of Akt , and the PI3K inhibi-tor could inhibit HO-1 expression.Conclusion: MALP-2 can induce THP-1 cells expression of HO-1 through TLR2,6/c-Src/PI3K pathways .关键词
支原体巨噬细胞活化脂肽2/血红素氧合酶-1/单核细胞Key words
Macrophage-activating lipopeptide-2/Hemeoxygenase-1/Monocyte分类
医药卫生引用本文复制引用
游晓星,马小华,刘良专,曾焱华,朱翠明,何军,蒋传好,吴移谋..支原体脂肽经TLR2，6／c-Src／PI3 K通路诱导单核细胞表达血红素氧合酶-1[J].中国免疫学杂志,2014,(5):587-590,4.基金项目
本文为国家自然科学基金（31000091，81072418）、湖南省高校科技创新团队资助项目（湘教通［2010］212号）、湖南省研究生培养创新基金资助项目（湘教通〔2008〕249号）。 （）
