中国免疫学杂志Issue(5)：639-643,5.DOI:10.3969/j.issn.1000-484X.2014.05.015
抗人IL-6 Rβ（gp130）单克隆抗体对IL-6信号分子调控浅析
Regulatory effect of anti-IL-6Rβ(gp130) mAb on IL-6 signaling pathway
摘要
Abstract
Objective:To analyse the biological function of anti-IL-6Rβ(gp130) monoclonal antibody and its regulatory effect on IL-6 signaling.Methods:Biological characteristics of anti-IL-6Rβ(gp130) mAb were assessed by Western blot analysis, capture ELISA and peptide ELISA .The phosphorylation of STAT 3 was tested by Western blot analysis in IL-6-stimulated U266/RA-FLS/RA-PBMC with or without anti-IL-6Rβ(gp130) mAb treatment.Results:3 strains of mouse anti-human gp130 mAb were with high affini-ty and different binding epitopes , the kaff of 10A1 was 2.62E-10.In U266, RA-PBMC and RA-SFMC, IL-6 signaling highly activated STAT3 which could be inhibited by anti-gp130 mAb.Conclusion: Anti-IL-6Rβ( gp130 ) mAb might have different binding epitopes and could affect IL-6 stimulated phosphorylation of STAT3, which provides a preliminary experiment for analyse the correlation of IL-6 signaling and RA .关键词
IL-6/gp130/抗gp130单克隆抗体/STAT3/RAKey words
IL-6/gp130/Anti-IL-6Rβ(gp130) monoclonal antibody/STAT3/RA分类
医药卫生引用本文复制引用
周晓薇,肖连波,陆梅生,张冬青,苗平,陈呢喃,赵蓉,钱柳,余奇文,张继英,许荣,何东仪..抗人IL-6 Rβ（gp130）单克隆抗体对IL-6信号分子调控浅析[J].中国免疫学杂志,2014,(5):639-643,5.基金项目
本文受国家自然科学基金（ No．31270963）和上海市科委自然基金（11ZR1427000）资助。 （）
