中国免疫学杂志Issue(5)：651-653,3.DOI:10.3969/j.issn.1000-484X.2014.05.018
7型腺病毒受体同源性多肽的筛选和研究
Screening and research of homologous peptide with adenovirus receptor
摘要
Abstract
Objective:To screen the binding peptide against adenovirus type 7(Ad7) and evaluate the relevance with the ade-novirus receptor .Methods:Binding peptide against Ad 7 was screened by panning the phage display 12 peptides library .The antibody against the selected peptide was prepared and was used to study the binding to the membrane by immunofluorescence technique .Re-sults:Using Ad7 as the target protein , GTS09 peptide was selected from the phage display 12 peptides library by biopanning .GTS09-phage complex could significantly bind Ad 7, with the affinity constant up to 1.93 ×1010 L/mol;at the same time, immunofluorescence showed that antibody of GTS09 could specifically bind to membrane of 293 cell.Conclusion: Antibody against GTS09 peptide could specifically bind to membrane of 293 cell,which shows that the peptide may presumably have homology with the cell receptors of Ad 7.关键词
7型腺病毒/受体/多肽/免疫荧光Key words
Adenovirus type 7/Receptor/Peptide/Immunofluorescence分类
医药卫生引用本文复制引用
许元生,田新贵,刘铭龙,李晨阳,周荣..7型腺病毒受体同源性多肽的筛选和研究[J].中国免疫学杂志,2014,(5):651-653,3.基金项目
本文为中国博士后基金（2012M511780）。 （）
