中国免疫学杂志Issue(5)：677-680,4.DOI:10.3969/j.issn.1000-484X.2014.05.024
骨髓间充质干细胞对Ⅰ型糖尿病大鼠淋巴细胞增殖的调节作用
Regulation of bone marrow mesenchymal stem cells on lymphocyte proliferation of type Ⅰdiabetic rats
郑贵星 1李翊泉 1魏小平 2吴颉 2黄俊3
作者信息
- 1. 广州医科大学附属第一医院输血科，广州510120
- 2. 广州医科大学附属第一医院检验科，广州510120
- 3. 广州医科大学病原生物学与免疫学教研室，广州 510180
- 折叠
摘要
Abstract
Objective:To investigate the regulation mechanisms of the bone marrow mesenchymal stem cells on lymphocyte pro -liferation of type I diabetic rats .Methods:The rat bone marrow mesenchymal stem cells were isolated , cultured and identified and the effect on lymphocyte proliferation of type Ⅰdiabetic rat was observed by MTT assay , and analyze the CD 4 +CD25 +regulatory T cell ra-tio, cell cycle and apoptosis of type I diabetes rat by flow cytometric .Results:B and C groups was significantly lower than the absor-bance values of group A,the differences between the data were statistically significant (P<0.05), C group was significantly lower than group B absorbance values, the difference was significant (P<0.05);the CD4 +CD25 +regulatory T cells of B and C groups were sig-nificantly higher than group A, the differences of the data were statistically significant (P<0.05), the CD4 +CD25 +regulatory T cell ratio of C group significantly higher than that group B , the differences were statistically significant (P<0.05);the apoptosis levels of B and C groups were significantly higher than group A , the differences were statistically significant (P<0.05), the apoptosis levels of C group were significantly higher in group B , the differences were statistically significant (P<0.05).Conclusion:Bone marrow mesen-chymal stem cells can significantly inhibit lymphocyte proliferation of type Ⅰdiabetic rats, and it may regulate CD4 +CD25 +regulatory T cells, promote apoptosis, thereby affecting the immune function of T lymphocytes , and play its rejection.关键词
骨髓间充质干细胞/Ⅰ型糖尿病/大鼠/淋巴细胞Key words
Bone marrow mesenchymal stem cells/Type Ⅰdiabetic/Rat/Lymphocytes分类
医药卫生引用本文复制引用
郑贵星,李翊泉,魏小平,吴颉,黄俊..骨髓间充质干细胞对Ⅰ型糖尿病大鼠淋巴细胞增殖的调节作用[J].中国免疫学杂志,2014,(5):677-680,4.
