中国免疫学杂志Issue(6)：745-748,753,5.DOI:10.3969/j.issn.1000-484X.2014.06.006
B7-H4在实验性自身免疫性心肌炎小鼠中的表达
Expression of B7-H4 in experimental autoimmune myocarditis
摘要
Abstract
Objective:To observe the expression of B7-H4 in experimental autoimmune myocarditis (EAM).Methods:BALB/c mice were randomly divided into 2 groups:the control group and the experimental group.The mice of experimental group were injected with myosin to establish EAM models , while the mice of control group were injected with complete Freund 's adjuvant and normal saline.All the mice were killed separately at the 14th,21st,30th and 45th day for lymphocyte proliferation assay ,hematoxylin-eosin staining,immunohistochemical staining and real-time PCR.Results:The inflammation infiltration of heart was most serious at the 14th and 21st day,then it was gradually relieved with time;the results of lymphocyte proliferation assay and real-time PCR were similar to that of the inflammation infiltration of heart ,which were in high level at the 14th and 21st day,and they were both higher than that of the control group ( P<0.05 );B7-H4 protein were only detected in the experimental group ,and it was constantly expressed during the whole experiment on the endothelium of heart with myocarditis.Conclusion:B7-H4 participates in the progress of EAM ,and it may be a new way of studying the mechanism of myocarditis.关键词
B7-H4/共刺激分子/实验性自身免疫性心肌炎Key words
B7-H4/Costimulatory-molecules/Experimental-autoimmune-myocarditis分类
医药卫生引用本文复制引用
刘忠梁,李戈,宋燕燕,崔彬,苑海涛..B7-H4在实验性自身免疫性心肌炎小鼠中的表达[J].中国免疫学杂志,2014,(6):745-748,753,5.基金项目
本文受国家自然科学基金项目（81070188）资助。 （）
