中国免疫学杂志Issue(6)：754-758,5.DOI:10.3969/j.issn.1000-484X.2014.06.008
冠心舒通对脑缺血再灌注大鼠学习记忆能力及脑组织Bcl-2、Bax蛋白表达的影响
Effect of Guanxinshutong on cerebral ischemia reperfusion in rats learning and memory ability and brain Bcl-2,Bax protein expression
摘要
Abstract
Objective:Guanxinshutong on cerebral ischemia reperfusion ( I/R) in rats learning and memory ability and brain Bcl-2,Bax protein expression to explore the protection mechanisms of Guanxinshutong on cerebral I /R injury in brain tissue.Methods:Healthy Wistar rats for the study were randomly divided into sham operation group ,I/R model group,high dose Guanxinshutong (1 g/kg) and low dose Guanxinshutong (0.5 g/kg) group,n=15.cerebral I/R model was estabhished by using the left commom cartid artery ligation method,modeling each group were fed daily before saline ,saline,Guanxinshutong (1 g/kg) and Guanxinshutong (0.5 g/kg), continuous 7 days;3 d after water maze training while continuing administration ,continuous 7 d,the eight day ,Morris water maze test;after the test,the rats were sacrificed blood and brain tissue ,serum by ELISA of Bcl-2 and Bax protein levels change;using HE staining of rat brain pathology;using immunohistochemistry assay in rat brain organization of Bcl-2 and Bax protein levels changes.Results:Compared with the sham group ,Guanxinshutong high-dose group test latency slightly longer timers after platform and platform quadrant dwell decreased slightly ,serum and brain tissue slightly elevated levels of Bax protein ,Bcl-2 protein levels decreased slightly but not significantly different ( P>0.05 ) ,low-dose group Guanxinshutong through latency test in rats ,after the number of platforms and platform quadrant dwell time decreased , serum and brain tissue elevated levels of Bax protein , Bcl-2 protein content decreased significantly different(P<0.01),cerebral I/R model group was significantly prolonged latency test ,after the number of platforms and platform quadrant dwell time significantly reduced serum and brain tissue was significantly increased Bax protein ,Bcl-2 protein content decreased with significant differences ( P<0.01 ).Conclusion: Guanxinshutong can significantly improve spatial memory , and by reducing Bax protein content and increased Bcl-2 protein,inhibition of cerebral I/R injury of apoptosis,and thus the brain tissue I/R has a protective effect.关键词
Morris水迷宫/冠心舒通/脑缺血再灌注损伤/脑组织/Bcl-2家族的前凋亡蛋白/Bcl-2Key words
Morris-water-maze/Guanxinshutong/Cerebral-ischemia-reperfusion-injury/Brain-tissue/Bcl-2-family-proapoptotic-proteins/Bcl-2分类
医药卫生引用本文复制引用
汪伟,李冬梅,马洪喜..冠心舒通对脑缺血再灌注大鼠学习记忆能力及脑组织Bcl-2、Bax蛋白表达的影响[J].中国免疫学杂志,2014,(6):754-758,5.基金项目
本文为吉林省教育厅资助课题（2010268）。 （）
