中国免疫学杂志Issue(6)：759-762,4.DOI:10.3969/j.issn.1000-484X.2014.06.009
紫花地丁含药血清对巨噬细胞吞噬功能及TOLL样受体表达的影响
Effects of viola-containing serum on phagocytosis and TOLL-like receptor expression of macrophage
摘要
Abstract
Objective:To investigate effects of viola on macrophage TOLL-like receptor expression ,and tentatively explore the partial mechanism of viola intervention on macrophage function.Methods: Using viola water decoction lavage intervention in clean grade SD rats of conventional preparation containing serum ,In a certain concentration coculture with mouse peritoneal macrophages in 96-well plates.After 24 hours,cell phagocytosis activity was determined by neutral red uptake assay ,changes in expression levels of TLR-1,TLR-2,TLR-3,TLR-4,TLR-5 mRNAs were determined by RT-PCR.Results:Compared with the same concentration of normal serum group :(1 ) viola-containing serum group macrophage activity was significantly increased ( P<0.01 );( 2 ) Within certain concentration,viola containing serum group with increased or decreased expression levels of TLR -1,TLR-2,TLR-3 mRNA(P<0.05 or P<0.01 ) ,the expression level of TLR-4 was not significantly altered in each group ( P>0.05 ) ,and the expression level of TLR-5 was significantly increased in each group ( P<0.01 ).Conclusion:Viola-containing serum has obvious promoting effect on macrophage phag-ocytosis,the mechanism of which may be related to that this drug regulates and controls part of macrophage TOLL -like receptor expres-sion.关键词
紫花地丁/血清药理方法/巨噬细胞/TOLL样受体Key words
Viola/Serum-pharmacological-method/Macrophages/TOLL-like-receptors分类
医药卫生引用本文复制引用
张智伟,蔡琨,吴玛莉,于红红,冷泠,岳文鹏,田维毅..紫花地丁含药血清对巨噬细胞吞噬功能及TOLL样受体表达的影响[J].中国免疫学杂志,2014,(6):759-762,4.基金项目
本文为贵州省委组织部人才办特助项目（ TZJF-2009年39号）、贵州省科技厅国际科技合作项目（黔科合外 G 字［2010］7014号）、贵阳中医学院研究生创新计划项目（ZYYCX12015）。 （）
