中国免疫学杂志Issue(6)：774-778,5.DOI:10.3969/j.issn.1000-484X.2014.06.012
GM-CSF和EB病毒LMP2 A融合基因的表达及抗肿瘤特性研究
Expression and anti-tumor characterization of fusion gene GM-CSF and LMP2A of EB virus gene
摘要
Abstract
Objective:To construct GM-CSF and EB virus LMP2A fusion gene recombinant adenovirus and identificate it , using recombinant adenovirus to do immunologic study.Methods:With RT-PCR and Western blot to detect GC 2A gene expression of the recombinant adenovirus , with ELISA to analysis antibodies of vAd-GC2A stimulation in murine body , lactate dehydrogenase assay to detect the mouse cellular immunity effect , thereby the recombinant adenovirus immune effect was initially analysis.T-test method was used to do preliminary analysis and evaluation of the immune effect of recombinant adenovirus .Results:GC2A fragment amplified by PCR, the size was 1 961 bp and it was the expected.The results showed, the recombinant adenovirus vAd-GC2A proteins can be recognized by sera antibody of GM-CSF ( LMP2A); the results of ELISA showed that vAd-GC2A can stimulate the generation of antibodies.EBV-positive tumor cell killing rate (%) by CTL induced recombinant adenovirus was (66.7 ±6.9)%, at the same time the adenovirus type 5 wild strains and PBS were (24.3 ±2.5)%and(32.4 ±3.1)%only(P≤0.05).Conclusion:Fusion gene has been successfully constructed , vAd-GC2A in mice have humoral and cellular immune function.The data suggest that the vAd-GC2A should play a potent role in preventing the positive EB virus tumor.关键词
融合基因GC2A/抗肿瘤/细胞免疫/EB病毒Key words
Recombinant-gene-GC2A/Anti-tumor/Cellular-immune/EB-virus分类
医药卫生引用本文复制引用
薛庆节,赵强,司传平,陈廷..GM-CSF和EB病毒LMP2 A融合基因的表达及抗肿瘤特性研究[J].中国免疫学杂志,2014,(6):774-778,5.基金项目
本文受山东省卫生厅支持项目（2007HW035）、山东省自然科学基金项目（ ZR2012 HM037）和山东省高等学校青年骨干教师国内访问学者项目资助（2012年度）。 （）
