中国免疫学杂志Issue(6)：808-813,6.DOI:10.3969/j.issn.1000-484X.2014.06.019
抗TLR2抗体抑制小鼠金黄色葡萄球菌肺炎炎症反应
Anti-TLR2 antibody attenuates inflammatory response of Staphylococcus aureus pneumonia in mice
摘要
Abstract
Objective:To investigate the effects of the anti-TLR2 antibody blocking TLR2 signaling pathway on inflammatory response in Staphylococcus aureus pneumonia murine models.Methods: Sixty C57BL/6J mice were divided randomly into normal control,SA pneumonia,and anti-TLR2 antibody group,killed 3 and 8 days after inoculation respectively.Normal control mice inoculated sterile PBS intranasally ,SA pneumonia mice inoculated SA ,anti-TLR2 antibody group of mice injected with anti-TLR2 antibody by tail vein and then inoculated SA intranasally.At the predetermined point , the colony-forming units ( CFU ) of bacteria were higher , leukocytes and neutrophil percentage were counted in bronchoalveolar lavage fluid ( BALF ) , the concentrations of KC and IL-10 in BALF and serum were assayed by ELISA ,changes in pulmonary histopathology were observed with HE staining and TLR 2 expression was detected by immunohistochemical.Results:3 days after intranasal inoculation ,the concentrations of KC and IL-10 in BALF and serum was increased in SA pneumonia mice , pulmonary histopathology changes significantly in HE staining.Compared with SA pneumonia mice,the CFU of bacteria were higher,leukocytes count and neutrophil percentage ,the concentrations of KC in BALF and serum,as well as HE pathological scores were reduced significantly in anti-TLR2 antibody group mice ,while no significant difference in IL-10.8 days after intranasal inoculation , HE pathological scores of anti-TLR2 antibody group mice were significantly lower than SA pneumonia group mice ,the CFU of bacteria in BALF were not statistically different between those two groups.Conclusion:Anti-TLR2 antibody attenuates the production of inflammatory mediators and inflammatory cell infiltration in SA pneumonia mice .关键词
金黄色葡萄球菌/抗体/肺炎/炎症Key words
Staphylococcus-aureus/Antibody/Pneumonia/Inflammation分类
医药卫生引用本文复制引用
温顺航,林立,李昌崇,苏小燕,张慧玲,吴沪军..抗TLR2抗体抑制小鼠金黄色葡萄球菌肺炎炎症反应[J].中国免疫学杂志,2014,(6):808-813,6.基金项目
本文为浙江省自然科学基金重点课题（ No.LZ13H010001）和浙江省研究生创新科研项目（No.YK2011083）。 （）
