中国免疫学杂志Issue(6)：814-816,3.DOI:10.3969/j.issn.1000-484X.2014.06.020
食蟹猴实验感染HBV的血清学反应
Serological response infected with on cynomolgus monkeys
摘要
Abstract
Objective:To study the serological reactions in cynomolgus monkeys infected with hepatitis B virus ( HBV). Methods:To select 1 to 3 days old or adult healthy cynomolgus monkeys by artificial breeding to observe the virology screening in laboratory a month to confirmed healthy animals ,randomly divided into control group and infection group .Infection group vaccination serum HBV carriers 0.5 ml (HBV-DNA≥108 copies) single cages,observe each group behavioral changes daily after inoculation 1 to 12 weeks, each week to confirmed the degree of liver inflammation through the HBV-M, HBV-DNA, liver function and on the B-guided, liver tissue inflammation by routine HE staining .Results: Adult monkeys did not induce positive reaction after vaccination , there were three young monkeys appear HBsAg , HBcAb and 2 appear HBV-DNA reaction, ALT poison attack occurred in HBsAg-positive began to increase after one week , one month after the peak , which was 180 U/L, after gradually decreased , continuing a month later near normal .AST higher than a week after the normal reference values were flat curve , representing the peak ALT after a month later, HBsAg positive cynomolgus monkeys HE staining showed mild hepatitis partial liver tissue lesions .Conclusion:HBV-M, HBV-DNA, ALT, AST and liver histopathology after HBV infection have changed , this result showing that it's produce inflammation and induction the response of immune .关键词
食蟹猴/肝炎病毒/乙型/动物模型Key words
Cynomolgus-monkey/Hepatitis-virus/Hepatitis-B/Animal-model分类
医药卫生引用本文复制引用
王振常,梁增文,冷静,韦毅,黄晶晶,陈松林..食蟹猴实验感染HBV的血清学反应[J].中国免疫学杂志,2014,(6):814-816,3.基金项目
本文为广西科技基础平台建设项目（12-97-22）、广西高校科技创新平台（桂教科研［2013］8号）、广西教育厅重点学科实验室项目。 （）
