中国免疫学杂志Issue(7)：870-873,878,5.DOI:10.3969/j.issn.1000-484X.2014.07.002
T细胞亚群偏移与雌激素缺乏骨丢失相关性实验研究
Experimental study of correlation between T-cell paradigm and bone loss induced by estrogen deficiency
摘要
Abstract
Objective:To demonstrate the relationship between the Th 1/Th2/Th17/Treg paradigm and the bone loss induced by estrogen deficiency and looking for potential target for clinical treatment.Methods:30 BALB/c mice were divided randomly into the normal control group , the sham operation group , and the ovariectomy group.The serum estradiol ( E2 ) was assessed by ELISA.Bone mineral density (BMD) of thigh bone was measured with dual energy X ray absorptiometry.Meanwhile,the T-cell subsets (Th1:CD4+TNFα+, Th2: CD4+IL-4+, Th17: CD4+IL-17 A+, Treg: CD4+CD25+Foxp3+) in spleen lymphocytes were analyzed by flow cytometry.Results:Compared with the normal group and the sham operation group , both E2 and BMD in the ovariectomy group decreased significantly ( P<0.05 ).The percentage of Th 1 and Th17 subset increased while the percentage of Th 2 and Treg decreased significantly in ovariectomy mice compared with sham operation mice.Correlation analysis showed that BMD was positively related to E 2 level and the percentage of Th 2 and Treg subset;however ,BMD was negatively related to the percentage of Th 1 and Th17 subset ( P<0.05 ).Conclusion: Conclusion: T-cell paradigm was involved in the bone loss induced by estrogen deficiency.Modifying T-cell paradigm may become a potential target for reducing bone loss induced by estrogen deficiency .关键词
雌激素缺乏/T细胞亚群/骨代谢/骨丢失/相关性Key words
Estrogen deficiency/T-cell paradigm/Boss metabolism/Bone loss/Correlation分类
医药卫生引用本文复制引用
高秀莲,李霞,邢美云,王丽,郭钰琪,周宪宾,张巧凤,张洪海,姚成芳,姜国胜..T细胞亚群偏移与雌激素缺乏骨丢失相关性实验研究[J].中国免疫学杂志,2014,(7):870-873,878,5.基金项目
本文受国家自然科学基金项目（ No.81373670）、山东省自主创新成果转化专项（2013ZHZX2A0405）、山东省博士基金项目（ No． BS2009YY041）、山东省科技发展计划项目（No.2012GSF11840）、山东省自然科学基金项目（No.ZR2010HQ066）、山东省计生委项目（No.［2009］8）、山东省中管局项目（2013-216，2013-218）、山东省计生委项目（［2009］8，［2014］25）和济南市科技明星计划项目（济科合字2012）资助。 （）
