中国免疫学杂志Issue(7)：913-916,4.DOI:10.3969/j.issn.1000-484X.2014.07.011
大籽蒿花粉过敏原的分离、鉴定与纯化
Identification and purification of major allergens in Artemisia sieversiana pollen
摘要
Abstract
Objective:To isolate,identify and purify the Artemisia sieversiana pollen ,the mostly widespread pollen among the Artemisia pollens in China.Methods: Artemisia sieversiana extract was precipitated by saturated ammonium sulfate and then electrophoresed by SDS-PAGE.The molecular mass of each protein band was determined by gel media system.The primary allergen proteins were identified by Western blot.Allergen proteins were purified and identified by DEAE-cellulose DE-52 ion exchange chroma-tography ( IEC) and Western blot.Results: We isolated more than twenty protein bands from Artemisia sieversiana pollen extract , including the most abundant six bands whose Mr were 62 kD,57 kD,38 kD,29 kD,25 kD,14 kD espectively.The protein bands with Mr were 62 kD and 16 kD had the highest binding capacity with the specific IgE from Artemisia pollen allergic patients.The DEAE-cellulose DE-32 IEC was used to purify the primary allergen proteins with Mr 62 kD and 16 kD.Conclusion:The primary allergens of Artemisia sieversiana include the allergen proteins whose Mr are 62 kD,16 kD and the allergen of Mr 62 kD and 16 kD can be purified by chromatography.关键词
大籽蒿花粉/过敏原/SDS-PAGE/Western blot/离子交换层析Key words
Artemisia sieversiana/Allergen protein/SDS-PAGE/Western blot/Ion exchange chromatography分类
医药卫生引用本文复制引用
李兴永,肖小军,孙宏治,何韶衡,杨平常,刘志刚..大籽蒿花粉过敏原的分离、鉴定与纯化[J].中国免疫学杂志,2014,(7):913-916,4.基金项目
本文为国家自然科学基金（ No.811160129、81302553）、广东省高等学校国际暨港澳台科技合作创新平台项目（No.2012gjhz0009）、深圳市科技计划国际科技合作项目（ No.GJHZ20130408174112021）。 （）
