中国免疫学杂志Issue(8)：1088-1092,5.DOI:10.3969/j.issn.1000-484X.2014.08.018
糖类抗原19-9单抗制备及DAS-ELISA的建立
Preparation against carbohydrate antigen 19-9 monoclonal antibodies and estab-lishment of DAS-ELISA
摘要
Abstract
To prepare monoclonal antibody of carbohydrate antigen 19-9(CA19-9).Methods: Based on the titer test results of mouse ascites and its IC 50 values ,the mouse that prepare for fusion was identified.Positive monoclonal cell strains were established by cell fusing and screening.Monoclonal antibody from ascites was produced by peritoneal injection monoclonal cell , and then purified by octoic acid-ammonium sulfate precipitation method.After determine the protein concentrations by UV-spectrophotometry ,the monoclonal antibody against CA 19-9 was labelled with horseradish peroxidase.Based on antibody pairing test , DAS-ELISA method was established .To compared with abroad kit , analyzing performance of this method.Results: Three strains of monoclonal antibody were obtained.And the optimal working concentrations of mAb (ZJY3-1G9) ,as coated antibody,McAb(ZJY2-7F10),as HRP-IgG,were assured.Limit of detection was 26.4 U/ml.Linear range was 30-300 U/ml.By detecting patients with serum 33 , confirmed the correlation coefficient of r=0.950 4 , compared with abroad kit that measure simultaneously.Conclusion:Monoclonal antibody prepared for CA 19-9 can be used to develop a kit.关键词
CA19-9/单克隆抗体/DAS-ELISAKey words
CA19-9/Monoclonal antibody/DAS-ELISA分类
医药卫生引用本文复制引用
王云龙,翟晋豫,王继创,程蕾,李玉林,葛新杰,毛烈..糖类抗原19-9单抗制备及DAS-ELISA的建立[J].中国免疫学杂志,2014,(8):1088-1092,5.基金项目
郑州市领军人才资助项目（131PLJRC666）。 （）
