中国免疫学杂志Issue(10)：1297-1301,1308,6.DOI:10.3969/j.issn.1000-484X.2014.10.001
中国湖北地区健康人群RAC1基因多态性的测定与分析
Analysis of RAC1 gene polymorphism in Hubei Chinese healthy population
摘要
Abstract
Objective:To establish a method for the detection of 8 single nucleotide polymorphisms (SNPs) of RAC1 gene,and to analyze the genotypic and allelic distributions of the 8 SNPs in healthy Chinese Han population.Methods:The real-time fluorescence TaqMan-MGB probes allele classification technology was used for the determination of the 8 SNPs in 150 cases of healthy volunteers in Hubei China ,and the results were validated by direct gene sequencing.Results: The method established in this study can accurately screen the genotypes of the 8 SNPs of human RAC1 gene.Highly linkage disequilibriums were found between rs 10951982 and rs9374 , rs702482 and 836488 ,respectively.All the 8 sits were in accordance with Hardy-Weinberg equilibrium.Six Tag-SNPs were selected by Heploview software:rs10951982 ,rs6954996 ,rs6951997 ,r1s2977 ,rs702482 and rs702483.The MAFs of the 8 SNPs in our study were close to the MAFs in CHB and Asian in Hap Map database .Significant distribution differences were also observed in different races.Conclusion:No significant differences are observed in this study in healthy Chinese Han population.But differences are found when compared with the data of other races in Hap Map.关键词
Rac1/单核苷酸多态性/基因测序/健康湖北人群Key words
Rac1/Single nucleotide polymorphisms/Genetic sequencing/Healthy Hubei Han population分类
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周嘉黎,刘亚妮,沈如飞,杨春晓,罗小梅,张玉,师少军..中国湖北地区健康人群RAC1基因多态性的测定与分析[J].中国免疫学杂志,2014,(10):1297-1301,1308,6.基金项目
国家自然科学基金项目（ No.81273591）、湖北省自然科学基金资助项目（ No.2009CDB380）和中央高校基本科研业务费专项资金资助（ No.2011JC039）。 （）
