中国免疫学杂志Issue(10)：1320-1324,1329,6.DOI:10.3969/j.issn.1000-484X.2014.10.005
结核分枝杆菌PhoPR双组分系统与不同毒力结核分枝杆菌致病性的相关性研究
Correlation study between Mycobacterium tuberculosis PhoPR two-component system and pathogenicity of different virulent Mycobacterium tuberculosis
摘要
Abstract
Objective:To explore the correlation between Mycobacterium tuberculosis PhoPR two-component system and the pathogenicity of different virulent MTB by analysing the expression levels difference of PhoP gene and PhoR gene in BCG ,H37Ra, H37Rv and XJ-MTB respectively.Methods:Total RNA extracted from four different virulent MTB strains and the integrity of total RNA were identified by using agarose gel electrophoresis.The expression of PhoP gene and PhoR gene were quantified by using SYBR Green I FQ-PCR.The expression levels difference of these genes were compared in different virulent MTB strains .Results: The relative expression levels of PhoP gene in between four different virulent MTB strains from high to low were XJ -MTB(9.05),H37Rv(1.00), H37Ra(0.25),BCG(0.08) respectively ,and the expression levels difference of PhoP gene were statistically significant in different virulent MTB strains ( P<0.05 );the relative expression levels of PhoR gene in four different virulent MTB strains from high to low were XJ-MTB(5.72),H37Rv(1.00),H37Ra(0.18),BCG(0.07) respectively,and the expression levels difference of PhoR gene were sta-tistically significant in different virulent MTB strains ( P<0.05 ).The expression levels of PhoP gene and PhoR gene at XJ-MTB were statistically significant difference compared with BCG ,H37Rv,H37Ra respectively (P<0.05);the expression levels of PhoP gene and PhoR gene at H37Rv were statistically significant difference compared with BCG ,H37Ra respectively (P<0.05);the expression levels of PhoP and PhoR gene at BCG were not statistically significant difference compared with H 37Ra (P>0.05).Conclusion:Significant expression levels difference of PhoP gene and PhoR gene are confirmed in different virulent MTB strains .Therefore,the Mycobacterium tuberculosis PhoPR two-component system is correlated with the pathogenicity in different virulent MTB strains.关键词
结核分枝杆菌/致病性/PhoPR双组分系统Key words
Mycobacterium tuberculosis/Pathogenicity/PhoPR two-component system分类
医药卫生引用本文复制引用
邬博,何丽,王钊,张玉清,庄睿,李文娟,梁晨,樊超,张万江,吴芳,章乐,吴江东,张辉,张春军,曹旭东,朱彬,李瑞山..结核分枝杆菌PhoPR双组分系统与不同毒力结核分枝杆菌致病性的相关性研究[J].中国免疫学杂志,2014,(10):1320-1324,1329,6.基金项目
国家自然科学基金资助项目（No.81260261，81160192）和新疆生产建设兵团医药专项资金资助项目（No.2012BA022）。 （）
