中国免疫学杂志Issue(11)：1529-1532,4.DOI:10.3969/j.issn.1000-484X.2014.11.020
强直性脊柱炎患者外周血中 miR-17、miR-181、miR-106、miR-30和miR-495表达的分析
miR-17,miR-181,miR-106,miR-30 and miR-495 level differences in peripheral blood of patients with ankylosing spondylitis
摘要
Abstract
Objective:In order to investigate the role of microRNA in the pathogenesis of ankylosing spondylitis patients,we detected the peripheral blood of patients with ankylosing spondylitis in miR-17,miR-181,miR-106,miR-30 and miR-495 expression, thereby to study the role of microRNA regulation of clinical and diagnostic value in the pathogenesis of ankylosing spondylitis provide new ideas.Methods:Collection of patients with ankylosing spondylitis and normal peripheral blood,peripheral blood mononuclear cells were isolated and extracted PBMC small RNA,using primers specific stem-loop reverse transcribed into cDNA,and build a mature miR-17,miR-181,miR-106,miR-30 and miR-495 T-carrier,standard curve,the use of stem-loop method by Real-time PCR technology to detect miR-17,miR-181,miR-106,miR-30 and miR-495 expression level.Results: In this study,92 cases of clinical samples were collected,of which 61 patients with AS,normal 31 cases.By Real-time PCR detection showed that compared with normal subjects,there was upregulation of miR-106 (P>0.05) and miR-30 (P>0.05) in the peripheral blood of patients with ankylosing spondylitis;down-regulation were miR-181 ( P<0.05 ) , miR-495 ( P<0.05 ) and miR-17 ( P>0.05 ) , in which the miR-181 and miR-495 was statistically significant.Conclusion:Compared with normal, there are differences in the peripheral blood of patients with ankylosing spondylitis expression of miR-495 and miR-181,which may be targeted to regulate TLR-4,HLA-B,DVL,GSK/3βgenes,this may be found in the pathogenesis of ankylosing spondylitis studies provide new ideas,to become a new clinical diagnosis markers.关键词
miRNA/强直性脊柱炎/Real-time PCRKey words
miRNA/Ankylosing spondylitis/Real-time PCR分类
医药卫生引用本文复制引用
刘鑫,魏军,杨芝红,周林林,郑锡铭,徐广贤..强直性脊柱炎患者外周血中 miR-17、miR-181、miR-106、miR-30和miR-495表达的分析[J].中国免疫学杂志,2014,(11):1529-1532,4.基金项目
本文为国家自然科学基金资助项目（31160494）、2011年教育部“新世纪优秀人才支持计划”项目（ NCET 11-11023）和2013年第二批自治区科技支撑计划项目（宁科计字［2013］20号）。 （）
