中国免疫学杂志Issue(12)：1591-1595,5.DOI:10.3969/j.issn.1000-484X.2014.12.002
YKL-40调控支气管上皮分泌 IL-8对支气管平滑肌细胞增殖和迁移的影响
YKL-40-induced IL-8 expression from bronchial epithelium leads to bronchial smooth muscle proliferation and migration
摘要
Abstract
Objective:To investigate YKL-40-mediated inflammation in human bronchial epithelial cells and analyzed the soluble factors secreted by bronchial epithelial cells exposed to YKL-40 that were responsible for increasing proliferation and migration of primary normal human bronchial smooth muscle cells (BSMCs).Methods:YKL-40-induced inflammation was assayed in two human bronchial epithelial cells (BEAS-2B cell line and primary human bronchial epithelial cells ,namely HBECs).In addition,we treated BEAS-2B cells and HBECs with YKL-40,and added the conditioned culture media ( YKL-40-BEAS-2B-CM) and ( YKL-40-HBECs-CM) to BSMCs.The proliferation and migration of BSMCs were determined by premixed WST-1 cell proliferation reagent and QCM chemotaxis migration assay ,respectively.Results: Bronchial epithelial cells treated with YKL-40 resulted in a significant increase of IL-8 production,but have no effect about RANTES ,Eotaxin and TNF-α.YKL-40-BEAS-2B-CM and YKL-40-HBECs-CM induced IL-8 was found to further stimulate proliferation and migration of BSMCs ,and the effects were inhibited after neutralizing IL-8.Conclusion:Through investigating the interaction of airway epithelium and smooth muscle ,our findings implicate that YKL-40 may be involved in the inflammation of asthma by induction of IL-8 from epithelium,subsequently contributing to BSMCs proliferation and migration.Moreover, inhibition of IL-8 signaling is a potential therapeutic target for YKL-40-induced inflammation and remodeling of asthma.关键词
YKL-40/支气管上皮细胞/IL-8/支气管平滑肌细胞Key words
YKL-40/Bronchial epithelial cell/IL-8/Bronchial smooth muscle cell分类
医药卫生引用本文复制引用
肖琳,石昭泉,李兵,修清玉,唐昊..YKL-40调控支气管上皮分泌 IL-8对支气管平滑肌细胞增殖和迁移的影响[J].中国免疫学杂志,2014,(12):1591-1595,5.基金项目
本文受国家自然科学基金项目（ No．81370137）和2013年度上海市卫生系统优秀学科带头人培养计划项目（ No．XYQ2013075）的资助。 （）
