中国免疫学杂志Issue(12)：1606-1610,5.DOI:10.3969/j.issn.1000-484X.2014.12.005
重组质粒 pchIL-18-MAGE 转染树突状细胞疫苗体外杀伤肝癌细胞的研究
Study of killing effect on liver cancer cells by transfected dendritic cell vaccine of recombinant plasmid pchIL-18-MAGE in vitro
摘要
Abstract
Objective:To observe lethal effect on liver cancer cells by the DCs transfected with recombinant plasmid pchIL -18-MAGE.Methods:Cultivate dendritic cells in vitro ,and recombinant plasmid pchIL-18-MAGE was constructed which was transfected into DCs.Expression of IL-18 and MAGE-1 on the transfected DCs were detected by RT-PCR and Western blot.The diversity of DCs phenotypes after transfect were analyzed by flow cytometry.Different killing effects to the target cells by lymphocytes which were stimulated by DC and those by non-transfected DC were detected.IFN-γin the cellular supernatant of the transfected DCs was inspected by ELISA.Results:pchIL-18-MAGE transfected DCs expressed higher level of CD 83,CD1a,CD86,CD80,HLA-DR,and expressed as a mature DC phenotype.pchIL-18-MAGE group′s killing activity was the highest ( P<0.05 ) .Conclusion: The DC vaccine pchlL-18-MAGE can induce better killing effect compared with other experimental groups.关键词
疫苗/肝癌/树突状细胞/MAGE-1/IL-18Key words
Vaccine/Hepatocellular carcinoma/Dendritic cells/MAGE-1/IL-18分类
医药卫生引用本文复制引用
刁斌斌,李首庆,马寅芙,杨广民..重组质粒 pchIL-18-MAGE 转染树突状细胞疫苗体外杀伤肝癌细胞的研究[J].中国免疫学杂志,2014,(12):1606-1610,5.基金项目
本文为吉林省科技发展计划项目（No.200905192，200705449）。 （）
