中国免疫学杂志Issue(12)：1611-1615,5.DOI:10.3969/j.issn.1000-484X.2014.12.006
不同雄性淋巴细胞及其Exosomes过继转输改善妊娠丢失孕鼠胚胎发育的比较分析
Comparative study of adoptively transferring patrilineal lymphocytes and their Exosomes on improving fetation of mice with pregnancy loss
摘要
Abstract
Objective:To analyze the protective effects of adoptively transferring different patrilineal lymphocytes and their Exosomes ( Exo) on fetation of mice with pregnancy loss comparatively.Methods: The peripheral blood mononuclear cells ( PBMC) from healthy men and the splenocytes from BALB/c and DBA/2 male mice were induced in vitro ,and their Exo were isolated through sucrose gradient ultra-centrifugation combined with ultrafiltration.The mice of CBA/J (♀) mated with BALB/c (♂) were enrolled as control group of normal pregnancy ,and the CBA/J (♀) mated with DBA/2 (♂) as URSA of pregnancy loss experimental animal model.The mice in URSA group were randomly divided into each group with treatment through adoptively transferring , which were injected intravenously or subcutaneously with splenocytes or splenocytes -derived Exo from mated DBA/2,unmated DBA/2 or unrelated BALB/c,also PBMC-derived Exo from men,respectively.And then,the placenta volumes,rates of fetal absorption and pregnancy loss were calculated to observe the fetation of embryos.Results:Compared with the group of normal pregnancy ,the placenta volumes from URSA group decreased greatly ,and rates of fetal absorption and pregnancy loss elevated greatly ( all P<0.000 5 ).After transferring different sources of cells and their Exo through different injection ,the placenta volumes resumed to the level of normal pregnancy ,and the rates of fetal absorption and pregnancy loss decreased significantly ( all P<0.000 5 ).No differences were observed after treatment through injecting intravenously or subcutaneously ( all P>0.05 ).After transferring the Exo derived from either male mice or healthy men,the level of decreased fetal absorption rates were more than that in cellular-therapy groups ( all P<0.05 ).After transferring the Exo derived from men ,the level of decreased pregnancy loss rates were more than that in cellular -therapy groups and mice splenocytes-derived Exo group ( all P<0.05 ).Conclusion:Adoptively transferring patrilineal T lymphocytes and their Exo can greatly improve the fetation.Exo should become a non-cellular bio-remedy,which is expected to replace traditional immunotherapy of adoptively transferring lymphocytes.关键词
T淋巴细胞/Exosomes/过继转输/妊娠丢失/胚胎发育/小鼠Key words
Tlymphocytes/Exosomes/Adoptive transferring/Pregnancy loss/Fetation/Mice分类
医药卫生引用本文复制引用
崔澂,郝淑维,刘杰,郑文广,王雅卓,张斯斯,阎娟,吕微,李梅香..不同雄性淋巴细胞及其Exosomes过继转输改善妊娠丢失孕鼠胚胎发育的比较分析[J].中国免疫学杂志,2014,(12):1611-1615,5.基金项目
本文为全军医学科技“十二五”科研面上项目（CWS11J307）、国家发明专利（No.ZL200710062487.6）。 （）
